摘要
用两个非放射性标记的寡聚核苷酸探针(GGAT)4和(GTG)5制作BALB/c、C57BL/6J、DBA/2、C3H等4种近交系小鼠的DNA指纹图,比较了两种探针在近交系小鼠遗传检测应用中的重复性和稳定性。结果表明,两种探针对上述4种近交系小鼠产生的DNA指纹图的图带数均为8~12条,具有良好的多态性。品系内的平均DNA指纹图相似系数(-x)在0·92~1·00的范围内,具有相同指纹图的概率(P)均在0·31以上,极显著地高于品系间的相似系数(0·22~0·39)和相同指纹图的概率(P<1·07×10-4)。说明(GGAT)4和(GTG)5两种寡聚核苷酸探针均可用于制作近交系小鼠的DNA指纹图,以对其进行遗传检测。用两种不同的探针进行DNA指纹分析,可以检出基因组中更多的个体特异性信息,结果更加可靠。
This study aimed to draw the DNA fingerprints of BALB/c, C57BL/6J, DBA/2, C3H inbred strain mice generated by two different non-radioactive labeled oligonucleotide probes (GGAT)4 and (GTG)5, and compared the reproducibility and stability of these two probes used in DNA fingerprinting for genetic monitoring. The results showed that there were 8 - 12 distinguishable bands on every DNA fingerprinting map generated by two probes and each map had polymorphism. The similarity coefficient (x) within breeds was 0.92 - 1.00, and the probability of an identical DNA fingerprinting (P) was above 0.31 ,significantly higher than that between breeds (x^- = 0.22 - 0.39, P 〈 1.07 × 10^-4 ) . It was indicated that the oligonucleotide probes (GGAT)4 and (GTG)5 could be used in DNA fingerprinting for genetic monitoring in inbred strain mice. Meanwhile, when we made DNA fingerprinting with two different probes, more individually specific information could be detected and the results were more reliable.
出处
《动物学杂志》
CAS
CSCD
北大核心
2007年第4期28-32,共5页
Chinese Journal of Zoology