桂枝茯苓丸对荷瘤鼠Fas表达的影响

Effect of GFW on the Fas Protein Expression in the S_(180) Bearing Tumor Mice

目的观察桂枝茯苓丸对S180荷瘤鼠的抑瘤作用及对脾脏中T淋巴细胞凋亡相关蛋白Fas的影响。方法计算抑瘤率,流式细胞仪测定正常组、模型组、中药组、环磷酰胺组脾脏中T细胞Fas表达。结果模型组、环磷酰胺组脾脏中T细胞Fas表达升高,与正常组相比,差异有统计学意义(P<0.01)。中药组Fas表达与模型组相比,差异有统计学意义(P<0.01),与正常组比较差异无统计学意义(P>0.05)。结论桂枝茯苓丸可通过抑制T淋巴细胞凋亡调节机体的细胞免疫。其机制可能与桂枝茯苓丸能调节脾脏中T细胞Fas表达密切相关。

Objective To explore the antitumor effects and the effect of GFW on the Fas expression in spleen T - lymphocyte of the tumor - bearing mice. Methods The S180 mice sarooma model was used to detective the inhibiting effects of GFW ,the Fas expression in spleen T - lymphocyte between the normal group , the model group, the GFW group and the CY group were analyzed by the flow cytometry. Results For S180 sarcoma ,the inhibiting ratio of GFW is 38.93%. The protein expression of Fas showed to rise in the the model group and the CY group. There was significant difference between them and normal group （ P 〈 0.01 ）. The Fas expression was declined in the GFW group when compared with the model group（P 〈 0.01 ） and there was no significant difference between the GFW group and normal group（P 〉 0.05）. Conclusions GFW has the antitumor function and can adjust immune through inhibitor the apoptosis of T - lymphocyte . its mechanism maybe closely related with adjusting expression levels of the Fas expression in spleen T - lymphocyte to normal.