大承气汤修复MODS大鼠小肠深部肌间神经丛神经-Cajal间质细胞-平滑肌网络结构损伤的研究

Effects of Dachengqi decoction(大承气汤) on nerve-interstitial cells of Cajal-smooth muscle network of enteric deep muscular plexuses in rats with multiple organ dysfunction syndrome

目的:观察多器官功能障碍综合征(MODS)大鼠小肠深部肌间神经丛神经-胃肠道Cajal间质细胞(ICC)-平滑肌网络结构的形态学改变和大承气汤对其的修复作用,探讨大承气汤治疗MODS的机制。方法:将50只Wistar大鼠随机分为对照组、MODS组和大承气汤治疗组;采用免疫荧光双标记、激光扫描共聚焦显微镜和透射电镜观察MODS大鼠小肠深部肌间神经丛胆碱能神经-ICC-平滑肌网络结构损伤和大承气汤对其的修复作用。结果:MODS组小肠深部肌间神经丛胆碱能神经-ICC-平滑肌网络连接结构不完整,神经纤维末梢、ICC、平滑肌超微结构以及相互之间的联系结构明显损伤。大承气汤治疗组小肠神经-ICC-平滑肌网络结构比较完整,神经纤维末梢、ICC、平滑肌超微结构无明显损伤,相互之间联系结构较为完好。结论:大承气汤能通过修复MODS大鼠小肠神经-ICC-平滑肌网络结构损伤,改善胃肠运动障碍,治疗MODS。

Objective： To observe the repairing effects of Dachengqi decoction （大承气汤） on the damage in the network of cholinergic nerve -interstitial cells of Cajal （ICC）-smooth muscle network of enteric deep muscular plexuses in rats with multiple organ dysfunction syndrome （MODS）. Methods： Fifty Wistar rats were randomly divided into control group, MODS group and Dachengqi decoction group. The network of cholinergic nerve - ICC - smooth muscle network of deep muscular plexuses of small intestine was observed by using c -Kit and vesicular acetylcholine transtorter （VAChT） immunohistochemical double -staining with whole -mount preparation technique and confocal laser scanning microscopy and transmission electron microscope. Results： Compared with control group, the distribution and density of cholinergic nerves and ICC in the deep muscular plexuses （ICC - DMP） of intestine in MODS group were significantly decreased （P〈0. 01）, the network of cholinergic nerve -ICC -smooth muscle was disrupted; and the ultra -structural features of ICC- DMP, nerve, smooth muscle ceils were severely damaged. After treatment with Dachengqi decoction, the network of cholinergic nerve - ICC - smooth muscle was significantly recovered. Compared with MODS group, the distribution and density of cholinergic nerves and ICC -DMP in treatment group were significantly increased （Pal0. 01） ; and the ultra -.structural features of ICC - DMP, nerve, smooth muscle ceils were significantly recovered. Conclusion ： Dachengqi decoction can improve the gastrointestinal motility in MODS and the mechanism may be related to its effects on the distribution and the connection of the network of cholinergic nerve - ICC - smooth muscle of deep muscular plexuses in the intestine of rats with MODS.