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α-葡萄糖苷酶高产菌株诱变选育的研究 被引量:5

Screening of a Mutant Strain Producing Alpha-transglucosidase
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摘要 α-葡萄糖苷酶是生产异麦芽低聚糖的关键酶。本文以α-葡萄糖苷酶产生菌黑曲霉M-1为出发菌株,经UV、UV-LiCl复合诱变和60Co诱变选育,成功筛选出了一株遗传稳定性较好的高产菌株C-1,其产酶活力是出发菌株的2.7倍。有望将来应用于异麦芽低聚糖的生产中。 Alpha-transglucosidase plays a critical role in producing isomalto oligosaccharide. In the paper, A stable mutant strain A.nigerC-I producing alpha-transglucosidase was obtained by ^60Co, UV and UV- LiCI mutant experiments. The productivity of the Alpha-transglucosidase of the mutagenesis is 2.7 times higher than that of the wild strain A.niger M-I.The strain would be applied in the production of isomaltooligosaccharide in the future.
出处 《现代食品科技》 EI CAS 2007年第7期19-21,共3页 Modern Food Science and Technology
关键词 Α-葡萄糖苷酶 ^60CO 诱变选育 alpha-transglucosidase ^60Co mutation breeding
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二级参考文献1

  • 1北京大学生物系生物化学教研室.生物化学实验指导[M]人民教育出版社,1964.

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