GC法测定云威灵软胶囊中百里香酚和异丁酸百里香酯的含量研究

Determination of Thymol and Isobutyric Acid Thyme in Yunweiling Capsule with GC Method

目的：采用GC法测定云威灵软胶囊中百里香酚、异丁酸百里香酯的含量。方法：色谱柱：100％DIMETHYL POLYSILOX ANE石英毛细管柱，30mm×0．25mm（SGE公司），柱温：160℃，汽化温度：220℃，检测器温度：200℃。载气为氮气，柱前压：6磅／英寸。，分流比为50：1，检测器：FID。结果：结果表明百里香酚在0．6～3．8g,g范围内具有良好的线性关系，异丁酸百里香酯在0．8～4．5峭范围内具有良好的线性关系，加样回收率：百里香酚为：99．07％，异丁酸百里香酯为：99．42％。结论：该方法简单迅速，结果准确，精密度好，可用于云威灵软胶囊的质量控制。

Objective： The gas chromatography（GC） method was used to determine the content of thymol and isobutyric acid thyme in Yunwei Capsule. Mehtod ： The chromatography column was 100% DIMETHYL POLYSILOX ANE quartz capillary column with column temperature 160 ℃, vaporization temperature 220℃ and detector temperature 200 ℃. The carder gas was nitrogen, precolumn press was 6 pound/inch2, split ratio was 50 ： 1, detector： FID. Result： The result indicated that thymol had a good linear relationship at the range of 0.6 - 3.8 μg, So do isobutyric acid thyme at the range of 0.8 -4.5μg, the spotting recovery was 99.07% in thymol, 99.42% in isobutyric acid thyme. Conclusion： The method is simple, accurate with good precision, can be used to control Yunweiling capsule.