体外培养人眼结膜上皮细胞

The Culture of Human Conjunctival Epithelium Cells in Vitro

目的探索体外分离培养人眼结膜上皮细胞的方法。方法用组织块培养法培养人眼结膜上皮组织,分别种植于培养皿及处理过的羊膜上,培养2周,观察结膜上皮细胞的生长特性。结果接种在培养皿的结膜上皮约6d组织块之间可融合成膜状,细胞为单层上皮细胞。接种在羊膜上的结膜组织生长较慢,约2周组织块之间可互相连接,融合成膜状,细胞为复层上皮细胞。免疫组化鉴定,结膜上皮细胞CK13染色阳性。结论组织块培养法是人眼结膜上皮细胞培养的较好方法,种植在羊膜上可获得复层上皮细胞。

Objective To study the way of isolating and culturing conjunctival epithelium cells in vitro. Methods The human conjunctival epithelium cells were cultured by tissue inoculation, cells were explanted in culture dish and on the treated human amniotic membrane, seperately. The cell morphology and growth features were observed after 2 weeks cultivation. Results The human conjunctival epithelium explanted in culture dish connected with each other into membrane shape in 6 d, and cells were monolayer ones. The human conjunctival epithelium explanted on human amniotic membrane connected with each other into membrane shape in 2 weeks, and cells were multi - layer ones. All cultured cells showed positive in CK13 staining by indirect immunofluorescence staining. Conclusion Tissue inoculation is a good approach for conjunctival epithelium cells culture, and multi - layer cells can be gained if the tissues are explanted on the human amniotic membrane.