氟尿嘧啶/左旋聚乳酸生物可降解纤维支架载药体系构建与缓释效应

Fabrication and sustained release of fluorouracil-loaded biodegradable Poly(L-lactic acid)fiber scaffolds

目的研发一种可供机体埋植的长效的氟尿嘧啶载药纤维支架。方法有机相分离法制备纤维,扫描电镜(SEM)观察纤维形态,光学显微镜测定纤维直径,红外光谱分析(FTIR)和差示扫描热分析(DSC)鉴定药物载体结合状态并测定纤维中PLLA的结晶度,紫外分光光度法(UV)测定纤维的载药量以及体外释放。结果制备出微米级的载药纤维,载药量与载药效率均较高;药物与聚乳酸属于简单物理混合;相对应两种结构模式载药纤维呈现两种释放模式。结论b型结构纤维适合于开发成长效的在位埋植载药纤维支架。

Purposes To develop a kind of long term released biodegradable and implantable fluorouracil-loaded PLLA fiber scaffold. Methods Fibers were fabricated by organic phase separation method. The structure of the fiber was observed by SEM and the state of the drug dispersed in the polymer and the crystallinity of the fiber was measured by DSC and FTIR analysis, respectively. The drug-loaded content and in vitro release profiles were determined by UV analysis. Results The fibers in micrometer range with high drug-loaded content and efficiency were successfully fabricated. The drug was dispersed in the polymer without change and the scaffold is just a simple physical complex of drug and polymer. The structures of the fiber will determine the drug release models of the scaffold. Conclusion The fibers with b type structure will be a good long term released biodegradable and implantable scaffold.