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人胚胎胰腺组织干细胞的分离鉴定和纯化 被引量：1

Identification and purification of human fetal pancreatic stem cells
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摘要 分离人胚胎胰腺组织中的胰岛细胞于体外培养,应用免疫细胞化学及RT-PCR的方法对其中的胰腺干细胞进行鉴定,并应用免疫磁珠的方法纯化其中的干细胞。结论是人胚胎胰腺组织中存在一定比例的干细胞,可以作为体外向胰岛素分泌细胞诱导分化的细胞来源。 Pancreatic islet cells were isolated from human fetal pancreatic tissue and identified through immunocytochemical methods and RT-PCR. Then pancreatic stem cells were purified using nestin antibody-conjugated magnetic beads. In conclusion, there are a certain proportion of pancreatic stem cells existing in human fetal pancreatic tissue, and they could be the resources of cell induction and differentiation into insulin producing cells.
作者 葛焕琦 蔡寒青 门秀丽 许世清 潘焕峰 娄晋宁
机构地区 吉林大学第二医院内分泌科 卫生部中日友好医院临床医学研究所 吉林市第二中心医院内分泌科
出处 《中国糖尿病杂志》 CAS CSCD 北大核心 2007年第7期437-439,共3页 Chinese Journal of Diabetes
基金 国家"863"基金(2002AA205061) 吉林省科委基金(200505124)资助项目
关键词 胰腺干细胞 糖尿病 诱导分化 胰岛移植 Pancreatic stem cell Diabetes mellitus Differentiation Islet transplantation
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献6

  • 1Shapiro AM,Lakey JR,Ryan EA,et al.Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.N Engl J Med,2000,343:289-290.
  • 2Zulewski H,Abraham EJ,Gerlach MJ,et al.Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine,exocrine,and hepatic phenotypes.Diabetes,2001,50:521-533.
  • 3Atouf F,Choi Y,Fowler MJ,et al.Generation of islet-like hormone-producing cells in vitro from adult human pancreas.Cell Transp,2005,14:735-748.
  • 4Ramiya VK,Maraist M,Arfors KE,et al.Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells.Nat Med,2000,6:278-282.
  • 5Gradwohl G,Dierich A,LeMeur M,et al.neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas.Proc Natl Acad Sci USA,2000,7:1607-1611.
  • 6Bonner-Weir S,Taneja M,Weir GC,et al.In vitro cultivation of human islets from expanded ductal tissue.Proc Natl Acad Sci USA,2000,97:7999-8004.

同被引文献14

  • 1Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000; 343: 230-238.
  • 2Merani S and Shapiro AM. Current status of pancreatic islet transplantation. Clin Sci (Lond). 2006; 110(6): 611-625.
  • 3Calne R. Cell transplantation for diabetes. Phiosl. Trans. R. Soc. Bio Sci, 2005, 360(1461):1769-1774.
  • 4Pipeleers D, in't Veld PI, Maes E, et al. Glucose-induced insulin release depends on functional cooperation between islet ceils. Proc Natl Acad Sci U S A, 1982; 79(23):7322-7325.
  • 5Ling Z, Hannaert JC, Pipeleers D. Effect of nutrients, hormones and serum on survival of rat islet beta cells in culture. Diabetologia, 1994; 37(1):15-21.
  • 6Bonner-Weir S, Taneja M, Weir GC, et al. In vitro cultivation of human islets from expanded ductal tissue. Proc Natl Acad Sci USA, 2000, 97(14):7999-8004.
  • 7Jiang FX, Cram DS, DeAizpurua HJ, et al. Laminin-1 promotes differentiation of fetal mouse pancreatic β-cells. Diabetes, 1999, 48(4):722-730.
  • 8Pamaud G, Hammar E, Rouiller DG, et al. Blockade of betal integrin-laminin-5 interaction affects spreading and insulin secretion of rat beta-cells attached on extracellular matrix. Diabetes, 2006, 55(5): 1413-1420.
  • 9Kaido T, Yebra M, Cirulli I, et al. Regulation of human β-cell adhesion, motility, and insulin secretion by collagen Ⅳ and its receptor α1β1. J Biol Chem, 2004, 279(51):53762-53769.
  • 10Hammar E, Parnaud G, Bosco D, et al. Extracellular matrix protects pancreatic β-cell against apoptosis. Diabetes, 2004, 53(8):2034-2041.

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中国糖尿病杂志
2007年 第7期

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