砷致小鼠脂质过氧化及锌、硒对其的干预研究

An intervention study of zinc and selenium on arsenic induced lipid peroxidation in mice

目的:探讨砷对小鼠血清及肝脏脂质过氧化的影响及锌、硒对其的干预作用。方法:采用亚慢性毒性实验方法,实验结束后分别测定小鼠血清、肝脏组织中脂质过氧化产物丙二醛(MDA)和谷胱甘肽S-转移酶(GST)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力,同时观察锌、硒干预后对染砷小鼠体内抗氧化水平的影响。结果:随着砷染毒剂量的增加,小鼠血清及肝脏组织中MDA和GST含量显著增加,SOD、GSH-Px活性显著降低,且呈剂量-效应关系。锌、硒能抑制MDA和GST生成量,提高SOD、GSH-Px活性。结论:砷可致机体脂质过氧化,锌、硒可拮抗其脂质过氧化作用。

Objective： To investigate the effect of arsenic on lipid peroxidation in mice liver and observe the intervention effect of zinc and selenium supplement. Methods： Subchronic toxicity experiment was applied. Then the mice organism and serum contents of lipid peroxides degradation product malondialdehyde （MDA）, the contents of glutathione S-transferase （GST）, the total superoxide dismutase （SOD） activities and glutathione peroxidase （GSH-Px） activities in serum and liver were determined individually. Meanwhile, study the influence of anti-oxidation activity in mice exposured to arsenic with zinc and selenium supplement. Results： The results showed that with the increases of exposure levels of arsenic,the contents of MDA and GST significantly increased and the activities of SOD and GSH-Px significantly decreased and showed a dose-effect manner. Zinc and selenium could decrease the contents of MDA and GST and increase the activities of SOD and GSH-Px significantly. Conclusion： The arsenic could induce lipid peroxidation in mice and zinc and selenium could inhibit the lipid peroxidation result in exposed to arsenic.