BRCA1和BRCA2蛋白在新疆维吾尔族女性乳腺良恶性疾病中的表达与意义

The significance and the expression of BRCA1 and BRCA2 in breast carcinoma and benign lesions in Xinjiang Uygur women

目的:研究BRCA1和BRCA2在新疆维吾尔族女性乳腺良恶性疾病中的表达及其临床意义。方法:应用组织芯片技术及免疫组织化学方法检测维吾尔族乳腺癌及乳腺良性病变组织中BRCA1和BRCA2的表达。结果:乳腺癌组织中BRCA1和BRCA2的阳性表达率分别为60.16%(77/128)和56.91%(70/123)。30例乳腺良性病变中BRCA1和BRCA2阳性表达率分别为80.00%(24/30)、83.33%(25/30)。乳腺癌组织中BRCA1及BRCA2的表达低于乳腺良性病变组织(P<0.05)。其在乳腺癌中的阳性表达部位除细胞核外,也见于胞质,而在乳腺良性病变中只在细胞核中表达。年龄及淋巴结转移情况不同的乳腺癌患者其BRCA1表达有统计学差异,而在肿瘤的大小、组织学分型及绝经情况均不同的患者间无统计学差异。BRCA2表达在组织学分型、肿块的大小、发病年龄、淋巴结转移情况及绝经情况均不同的患者间的差异均无统计学意义。结论:BRCA1和BRCA2蛋白的检测有可能将作为估计乳腺癌生物学行为和预后的新指标。乳腺癌-乳腺良性病变组织微阵列的建立使蛋白产物的筛选工作简便、快捷。

Objective： To study the expression and the clinical significance of BRCA1 and BRCA2 in the breast carcinoma and breast benign lesion in Xinjiang Uygur women. Methods： BRCA1 and BRCA2 were examined by tissue microarray technology and immunochemical method in breast carcinoma tissues and lesion tissues in Xinjiang Uygur women. Result： The expressive rates of BRCA1 and BRCA2 in breast cancer were 60. 16%（77/128）, 56.91% （70/123）. The expressive rates of BRCA1 and BRCA2 in breast benign lesions were 80 %（24/30）, 83.33%（25/30）. The expressive rates of BRCA1 and BRCA2 in breast cancer were lower than those in breast benign lesions （P 〈0.05）. Positive location in breast carcinoma cells located in the cell cytoplasm, or presented in the nucleus and cytoplasm, but only expression in the nuclear in the benign lesions, the expression of BRCA1 was significant with the age of patients and the axillary nodal metastases （P 〈0.05）. but the expressive difference of BRCA1 is insignificant with the size of tumor, the histological type and whether the patient presented the menopause （P 〉0.05）. The expression of BRCA2 was insignificant correlation with the size of.tumor, axillary nodal metastases, the age, the histological type and having or not menopause （P 〉0.05）. Conclusion： Detecting of BRCA1 protein and BRCA2 will became the new factor in estimating the biological behavior and the prognosis of breast carcinoma in Xinjiang Uygur women. In addition, the facilitates of tissue microarray of breast carcinoma-breast benign lesions is convenient and quick to detect the protein.