豚鼠慢性化脓性中耳炎鼓室硬化模型

Establishment of an animal model with tympanosclerosis in guinea pigs with chronic otitis media

目的研究建立慢性化脓性中耳炎鼓室硬化动物模型豚鼠的方法,为慢性化脓性中耳炎鼓室硬化的动物实验研究提供一种有用的动物模型。方法采用小鼠腹腔连续传代3次增强毒力的金黄色葡萄球菌,采取反复中耳腔注射的方法,制备豚鼠慢性化脓性中耳炎动物模型,了解化脓性中耳炎对听阈的影响及是否导致鼓室硬化。结果将钙化斑及局部骨化作为鼓室硬化的标志,接种细菌后1周就可观察到中耳粘连的出现,接种后2周出现钙化斑等鼓室硬化灶,听力较对照耳明显下降。观察到硬化灶最常见的部分是下鼓室,其次是鼓室上隐窝、上鼓室、鼓膜、听骨链间隙和咽鼓管鼓口。结论豚鼠慢性化脓性中耳炎鼓室硬化动物模型病理变化与人慢性化脓性中耳炎鼓室硬化类似,是研究慢性化脓性中耳炎鼓室硬化较好的动物模型。

To establish the optimal animal model of tympanosclerosis. METHODS Staphylococcus were inoculated into the middle ear of guinea pigs, and the guinea pigs were sacrificed periodically. The changes of their middle ears were observed by light microscope, and the hearing threshold of the animals were measured. RESULTS All the inoculated ears showed different degree of mucosal adhesions after 7 days. The most common site of tympanosclerosis was hypotympanum, followed by the anterior epitympanum, the attic, the drum, the interossicular spaces, and the tubal orifice. CONCLUSION The pathological changes of the middle ear in guinea pigs with chronic otitis are similar to those of human being. It is a favorite animal model for researching on chronic otitis media