栓塞大鼠大脑中动脉对海马结构内星形胶质细胞的形态学影响

The effects of occlusion of middle cerebral artery on the morphology of astrocyte in hippocampal formation

目的:探讨栓塞大鼠大脑中动脉是否可以引致海马结构内星形胶质细胞的形态学改变。方法:用免疫组织化学方法显示栓塞大脑中动脉大鼠、假手术大鼠和对照大鼠海马结构内的GFAP阳性的星形胶质细胞的形态和数量以及GFAP表达,对比组间差异,寻找梗塞对远离缺血中心区的海马结构内星形胶质细胞的影响。结果:栓塞大鼠大脑中动脉可以引致远离缺血中心区的海马结构内星形胶质细胞数量增多、体积增大和GFAP表达增加。结论:脑缺血损伤引起的胶质细胞激活不仅限于缺血中心区和边缘区,远离缺血中心区的脑区也可以出现神经胶质细胞形态学改变,即胶质细胞激活现象。

Objectivc： To study the effects of occlusion of middle cerebral artery on the morphology of astrocyte in hippocampal formation in rat. Methods： Immtmocytochemistry were used to demonstrate GFAP positive cells in hippocampal formation from the control, sham and model groups, and compared differences of the shape, the positive number and the GFAP expression of astrocytes among three groups. ~： The cell population, cell volume and GFAP expression in hippocampal formation which is far from central ischemic area increased significantly in the model group compared with the control and the sham groups. Conclusions： Astrocyte activation induced by occlusion of middle cerebral artery not only occurs in the central and marginal isehemic areas, but also in the region which is far away from the ischernie region.