琥珀明胶和羟乙基淀粉注射液对大鼠肾缺血/再灌注损伤肾功能及血流动力学的影响

Effect of Gelofusion and 6% Hydroxyethyl starch on renal function and hemodynamics in SD rats duing the renal acute ischemia/resperfusion injury.

目的探讨输注不同血浆代用品琥珀明胶(血定安)或羟乙基淀粉注射液(贺斯)后,对大鼠肾缺血再灌注损伤肾功能及血流动力学的影响。方法建立SD大鼠肾缺血再灌注模型,并随机分为4组:假手术组(Ⅰ组)、生理盐水对照组(Ⅱ组)、血定安组(Ⅲ组)和贺斯组(Ⅳ组)。生化法检测大鼠血清肌酐(Scr)、尿素氮(Bun)、6-酮-前列腺素(6-keto-PGF1α)、血栓素B2(TXB2)水平并计算血栓素/6-酮-前列腺素(TXB2/6-keto-PGF1α)水平,Ⅱ、Ⅲ、Ⅳ三组大鼠监测术中血压及心率的变化情况。结果与Ⅰ组比较,Ⅱ、Ⅲ、Ⅳ三组肾动脉开放再灌注24 h后Scr、Bun、TXB2和TXB2/6-keto-PGF1α水平升高(P<0.01),与Ⅱ组比较Ⅲ、Ⅳ二组平均动脉压(MAP)水平升高(P<0.05)。结论血定安和贺斯对大鼠肾缺血再灌注损伤肾功能损害的影响没有显著性差异而在维持血流动力学稳定方面有明显作用。

Objective To investigate the effect of Gelofusion and 6% Hydroxyethyl starch on rats renal function and hemodynamics after the renal ischemia and repefusion. Methods 80 male healthy SD rats were randomly divided into 4 groups with 20 animals in each group： group Ⅰ recived no opration; group Ⅱ recived normal saline iv; group Ⅲ recived Gelofusion iv; group Ⅳ recived 6% Hydroxyethyl starch iv. blood samples were taken before ischemia and after 0.25 hour and 24 hour repefusion for determination of Bun,Cr,6 - keto - PGFIct and TXB2. Group Ⅱ,Ⅲ, Ⅳ need separate the thigh - arterial and constantly moniter the blood pressure and heart rate. Results In contrast with group Ⅰ , the levels of Scr, Bun,TXB2 and TXB2/6 - keto - PGF1α in group Ⅱ,Ⅲ and Ⅳ after 24 hour repefusion were higher significantly than that before ischemia （ P 〈 0.01 ） ; In contrast with group Ⅱ, the mean arterial pressure levels of group Ⅲ and Ⅳ were higher significantly （ P 〈 0.05 ）. Conclusion In the ischemia and reperfusion injury models of rats,the infusion of Gelofusion and 6% Hydroxyethyl starch do not cause murther injury to renal function and the infusion of Gelofusion and 6% Hydroxyethyl starch can make the hemodynamics more stable than Normal saline.