重度子痫前期患者母儿间HLA Ⅱ类抗原相容性的研究

Studies on the HLA class Ⅱ genes in patients with severe pre-eclampsia and their infants

目的:研究重度子痫前期患者母儿间HLA Ⅱ类抗原HLA-DRB1,-DQB1和-DPB1有无相容性或相斥性。方法:用多聚酶链反应-限制性片段长度多态性分析(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测31例重度子痫前期组母儿和34例正常妊娠母儿的HLA-DRB1,-DQB1和-DPB1的基因型和表型。计算母儿间不一致的HLA基因数和(或)抗原数,比较两组间的差异。结果:未发现重度子痫前期组母儿间存在明显的HLA-DRB1,-DQB1或-DPB1基因型或表型的显著相容或相斥。但是,重度子痫前期组母儿间HLA-Ⅱ类抗原基因型的相容性显著多于正常对照组,母儿间Ⅱ类抗原基因型完全相符的例数显著高于对照组(分别为4例和0例,P=0.046)。结论:母儿间HLA Ⅱ类抗原基因型相容性增加可能参与了重度子痫前期的发病。

Objective：To investigate if maternal-fetal compatibility or dissociation of HLA-DR,-DQ and -DP alleles exists or not in women with severe pre-eclampsia. Methods： HLA-DRBI ,-DQB1 and -DPB1 genotypes and phenotypes were determined by PCR-RFLP （polymerase chain reaction-restriction fragment length polymorphism ） method in 31 women with pre-eclampsia, their infants, and 34 normal fertile women and their infants. The number of mismatched genotypes and phenotypes between mothers and infants was counted. Comparison was made between patients and normal group. Results.No significant compatibility or dissociation of HLA-DRBI ,-DQB1 or -DPB1 genotype or phenotype was found between women with se- vere pre-eclampsia and their infants. The compatibility of HLA class Ⅱ genotypes between women with severe pre-eclampsia and their infants was significantly higher comparing to normal fertile women （ P = 0. 046 ）. The number of which the infant shared all of six HLA-DRB1 ,- DQB1 and -DPB1 alleles with the mother was significantly higher in pre-eclampsia group compared to normal fertile group （ 12.9% vs 0.0% ）. There was no significant difference of the number of incompatible HLA-DRB1 -DQB1 and -DPB1 phenotypes between pre-eclampsia group and normal fertile group. Conclusion：It is implied that women who conceive fetuses possessing totally compatible HLA class II genotypes with them tend to be at risk of developing preeclampsia.