原因不明复发性流产患者血清趋化因子RANTES与封闭效率的关系

The expression of the regulated upon activation normal T cell expressed and secreted in maternal serum and its correlation with mixed lymphocyte reaction-blocking effect in unexplained recurrent spontaneous abortion

目的:研究原因不明复发性流产(URSA)患者血清中趋化因子RANTES与夫妇间混合淋巴细胞反应封闭效率(MLR-BE)的关系,探讨RANTES、MLR-BE在URSA发病中的作用。方法:以URSA患者40例为URSA组,有正常生育史且无流产或早产等不良生育史的40例为对照组。(1)用酶联免疫吸附(ELISA)法,检测血清中RANTES水平;(2)用单向混合淋巴细胞反应封闭试验,在未加入与加入RANTES试剂情况下,分别检测血清中MLR-BE水平。将测得的MLR-BE分别设为MLR-BE1、MLR-BE2。结果:(1)URSA组RANTES水平明显低于对照组,两组差异有统计学意义(P<0.05);(2)URSA组MLR-BE1明显低于对照组,两组差异有统计学意义(P<0.05);URSA组MLR-BE2较MLR-BE1明显上升,两者差异有统计学意义(P<0.05);URSA组MLR-BE2与对照组差异无统计学意义(P>0.05);对照组MLR-BE2与MLR-BE1差异无统计学意义(P>0.05);(3)两组MLR-BE1与血清中RANTES水平呈正相关。结论:(1)URSA组RANTES、MLR-BE水平明显低于对照组,有助于URSA的早期诊断;(2)URSA组血清中RANTES与MLR-BE水平呈正相关,提示二者在URSA发病中可能起协同作用。

Objective：To investigate the regulated upon activation normal T cell expressed and secreted （RANTES） in serum and its correlation with mixed lymphocyte reactionblocking effect （MLR-BE） in unexplained recurrent spontaneous abortion （URSA）. Methods： Forty patients with URSA were studied, forty cases of fertile women were selected as normal group. RANTES was determined using enzyme linked immunosorbent assay （ELISA）. MLR-BE was tested by blocking test one-way mixed lymphocyte reaction,which performed in the absence and presence of recombinant human RANTES. Results： （ 1 ） The level of RANTES in URSA group was lower than that in normal group （ P 〈 0.05 ）. （2） In the absence of RANTES, the level of MLR-BE1 in URSA group was lower than that in normal group （ P 〈 0.05 ）. In the presence of RANTES, the level of MLR-BE2 was lower than MLR-BE1 in URSA group （ P 〈 0. 05）, the level of MLR-BE2 in URSA group had no difference with normal group （P 〉 0. 05）,the level of MLR-BE2 and MLR-BE1 had no difference in normal group （P 〉0.05）. （3） Significant positive liner correlation was revealed between RANTES and MLR-BE1 （P 〈 0. 05 ）. Conclusions： （ 1 ） The level of RANTES and MLR-BE in URSA group is significantly lower than those in normal group. They are useful in the diagnosis of URSA ; （2） A positive relationship between RANTES and MLR-BE is found in URSA group and normal group. This indicates that RANTES and MLR-BE play interdependant roles in the onset and development of URSA.