摘要
背景与目的:探讨不同染色时间、不同阅片时间及阅片人的熟练程度对微核识别率的影响。材料与方法:血样采自1名30岁健康男性。分离淋巴细胞并以RPMI1640培养,分2个照射组(1Gy、2Gy)和1个对照组,共3组。采用胞质分裂阻滞微核实验方法低渗制片,每组制备2张玻片,每张玻片滴制a、b2个计数点,并分2批在不同时间用Giemsa染色。6名对微核实验熟练程度不同的阅片人分别按要求阅片。结果:在统一计数标准的前提下,阅片人熟练程度导致的微核判读结果差异不显著(P>0.05);全部阅片人在不同时间对同一玻片a、b2个计数点的微核计数结果之间差异无统计学意义(P>0.05);不同时间染色标本的核分裂指数(NDI)、微核细胞数(MNed)、微核数(MNi)和核分裂指数(NDI)等结果之间差异无统计学意义(P>0.05)。结论:在本实验条件下,阅片人熟练程度、不同时间染色或阅片均不会对微核识别的判断造成明显影响。
BACK(ROUND & AIM:To study the effect on MN frequency identification caused by different skill level, staining time and scoring time. MATERIALS AND METHODS: A blood sample was collected from a healthy 30-year-old male. Lymphocytes was isolated and then cultured in RPMI medium in triplicates. Two of the 3 cultured groups were then exposed to 1Gy or 2 Gy gamma-rays and the other one was control. Parallel scoring spots (a and b) in same slide was made according to CBMN assay method, 2 simultaneously parepared slides of each group was stained by Giemsa at 2 different times, and then 6 scorers with different skill levels scored the MN frequency. RESULTS: Variation of MN frequency scored by scorers with different skill levels was not significant. The MN frequency scoring for spot a and b at different times showed no significant difference among different scorers. The observed endpoints, MNed, MNi, NPB and NDI, stained at different times, were not different significantly either. CONCLUSION: Skill level, staining time or scoring time did not show significant interference on estimation of the dose-effect relationship between MN frequency and radiation dose.
出处
《癌变.畸变.突变》
CAS
CSCD
2007年第4期331-334,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
微核试验
熟练程度
染色时间
阅片时间
micronucleus test
skill level
staining time
scoring time