瘦素在增殖性糖尿病视网膜病变中作用的研究

The Study of Leptin in Eyes of Patients with Proliferative Diabetic Retinopathy

目的探讨瘦素及其受体在增殖性糖尿病视网膜病变(PDR)形成发展中的作用。方法应用免疫组织化学技术检测增值性糖尿病视网膜病变(PDR)患者视网膜前膜及正常视网膜瘦素效应受体-长型受体的表达,并采用酶联免疫吸附技术测定PDR患者玻璃体液及正常人玻璃体液瘦素浓度。将结果进行统计学比较分析。结果PDR患者视网膜前膜组织有瘦素受体的表达,V期,VI期表达的阳性率之间无显著性差异;正常视网膜组织未见表达。PDR患者玻璃体液瘦素浓度比正常人显著性升高;V期,VI期PDR患者玻璃体液瘦素浓度无显著性差异。结论瘦素及其受体参与了DR的发病,并促进DR从非增殖期向增殖期进展。

Objective To study the action of leptin and leptin receptor（Lep-R） on the development and progress of proliferative diabetic retinopathy （PDR）.Mthods Immunohistological technique was used to detect the expression of long receptor-leptin effecting receptor on epiretinal membranes（ERM）of patients with proliferative diabetic retinopathy（PDR）.ELISA technique was uesd to test the concentration of leptin in vitreous both in patients with PDR and normal people.Results Leptin receptor is expressed on ERM of patients with PDR,no statistic meaning on positive rate between the fifth and sixth phase,The concentration of leptin is significantly increased in vitreous in patients with PDR compared with normal people.The diference has statistic meaning.The diference of concentration of leptin between the fifth and sixth phase on patrents with PDR has no statistic meaning.Conclusions Leptin and its receptor are involved in the development and progress of DR and cause the developing of DR from non-proliferative phase to proliferative phase.