盐酸纳洛酮对大鼠DBI合并SBI后c-fos基因表达及β-EP的影响

THE EFFECT OF NALOXONE HYDROCHLORIDE ON C-FOS GENE EXPRESSION IN BRAIN AND BETA-ENDORPHIN OF BLOOD PLASMA OF RATS WITH DBI AND SBI

目的:通过应用纳洛酮干预后,观察DBI合并SBI后血浆β-EP与c-fos基因表达的变化,进一步探讨血浆β-EP与c-fos基因在SBI中的作用。方法:70只雄性SD大鼠随机分为DBI合并SBI组(A组),DBI合并SBI后盐酸纳洛酮治疗组(B组),DBI后SBI前盐酸纳洛酮治疗组(C组);分别采用免疫组化及放免分析测定大鼠脑内c-fos蛋白与血浆β-EP的含量,并进行脑组织病理显微结构的观察。结果:B、C两组在各时间点的各项检测指标之间,均无显著性差异。B、C两组脑组织含水量在3h及24h时,均较A组明显升高(P<0.05);神经元损伤数量在24h及48h均较A组明显减少(P<0.05);伤后3h、24h及48h,B、C两组的c-fos基因表达均明显高于A组;伤后3h、24h,B、C两组的血浆β-EP含量也明显高于A组(P<0.05)。结论:盐酸纳洛酮可降低DBI合并SBI大鼠c-fos基因表达及血浆β-EP含量,降低死亡率及神经损伤程度,具有一定神经保护作用;DBI合并SBI后使用盐酸纳洛酮与DBI后预处理使用盐酸纳洛酮对SBI的疗效相仿。

Objective： All rats with diffuse brain injury （DBI） and secondary brain insult （SBI） were injected intraperitoneal with naloxone hydrochloride, then teat C- fos gene expression and changes of beta- endorphin, and analyze the effects and significance of C - fos gane and beta- endorphin in development of rats SBI. Method：All animals in treatment group were injected intraperitoneal with naloxone hydrochloride （1.0mg/kg） 15 minutes after diffuse brain injury or 15 minute before DBI, while all animals in control group were injected with 0.9% saline instead. Then the rats were tested for the motor and cognitive performance 24 hours after injuries. The water contents, the numbers of injured neurons were recorded, the beta - endorphin of blood plasma and the number of c-fos - positive cell were recorded at different intervals post - injuries. Results： The morality, the water contents in the frontal cortex, the beta - endorphin of blood plasma, the numbers of c - fos - positive cell and the thronged neurons of treatmental group were lower than that of control group （P〈0.05） . Conclusion： The results of this study indicate that naloxone hydrochloride provides remarkable protection against the damage exacerbated by secondary brain insults.