双侧颈总动脉结扎大鼠学习记忆能力的研究

STUDY OF THE ABILITY OF LEARNING AND MEMORY OF THE RATS WITH PERMANENTLY BILATERAL COMMON CAROTID ARTERY LIGATION

目的:观察2-VO模型SD大鼠的学习记忆行为学特征。方法:SD大鼠制备2-VO模型,造模1个月后利用Morris水迷宫检测模型大鼠空间学习记忆能力。结果:Morris水迷宫定位航行试验结果显示,模型组大鼠的平均逃避潜伏期(44.61±36.73)s显著大于假手术组(20.20±14.98)s和正常组(19.51±14.92)s(P<0.05)。大鼠空间探索试验中平台象限游泳时间与总游泳时间之比(tP/tT值),模型组tP/tT值(0.319±0.083)显著小于正常组(0.460±0.088)和假手术组(0.445±0.082),P<0.05。结论:2-VO模型大鼠空间学习记忆能力明显下降,是研究脑缺血后神经损伤及智能障碍的理想动物模型。

Objective： To study the ability of learning and memory of 2 - VO rats. Method： Model rats were performed by permanently bilateral common carotid artery ligation ：for 5 weeks. Morris water maze test was performed at the end of experiment. Results： The average escape latency of model group （44.61 ± 36.73） s was significantly longer than those of sham operate group （20.20 ± 14.98） s and normal group （19.51 ± 14.92 ） s during platform orientation test （P 〈 0.05 ） . tP/tT of model group （0.319 ± 0. 083） was much lower than those of sham operate group （0. 445 ± 0. 082 ） and normal group （0. 460 ± 0. 088） during petial probe test （P〈0.05） . Conclusion： With the ability of learning and memory of was obviously impaired, 2 - VO rat was a typical model for study of nerve injury and disturbance of intelligence after cerebral ischemia.