双探针实时荧光定量法检测HCV RNA抗原的临床意义

Clinical significance of detection of HCV RNA by using double probed real-time fluorescence quantitiative determinatior

目的建立双探针荧光定量HCV RNA检测方法,分析其定量检测HCV RNA的灵敏度和特异性以及HCV RNA含量与患者病情的相关性。方法选取100例抗HCV抗体阳性样本,包括慢性肝炎患者45例、肝硬化患者30例、肝癌患者25例,并选取献血员50例为对照,用双探针荧光定量法和两种商品荧光定量试剂方法进行HCV RNA检测。结果双探针荧光定量法阳性率为93%(93例);两种商品荧光定量试剂方法阳性率分别为84%(84例)和79%(79例)。结论双探针荧光定量提高HCV RNA检测的灵敏度和特异性,HCV RNA含量与丙型肝炎患者病情变化及严重程度相关。

Objective To establish real- time fluorescence quantitative reverse transcription polymerase chain reaction （RFQ- RT - PCR） for determianfion of HCV RNA and evaluatate the significance of RFQ - RT - PCR in determnianton of HCV RNA by using double - probes. Methods A total of 100 anti - HCV positive cases and 50 anti - HCV negative cases were determined with RFQ - RT- PCR by using double probe, and were also detected by other two HCV RNA quantification kits simultaneously. Results HCV RNA positive rate was 93% （93 cases） in RFQ- RT- PCR by using double- probes,while it was 84% （84 cases） and 79% （79cases） by using other two kits. Conclusion RFQ-RT- PCR assay using doubleprobes can enhance the sensifivity and specificity of HCV RNA detection. HCV RNA load may be associated with the progress and severity of disease.