苯诱导小鼠骨髓嗜多染红细胞微核的研究

Observation on the frequencies of micronucleus in bone marrow polychromatic erythrocyte of mice induced by benzene

目的探讨苯对小鼠体细胞遗传物质的影响。方法采用静式吸入法染毒,将50只动物随机分成阴性对照组、阳性对照组及0.3、0.6、1.2g/m33个剂量组,剂量组每隔24h染毒1次,每次2h,共15d,取胸骨骨髓作微核计数,分析受试小鼠骨髓嗜多染红细胞微核率。结果苯染毒低、中、高3个剂量组的微核率与阴性对照组比较,差异有统计学意义(P<0.05),并呈现剂量-反应关系。结论苯能诱导小鼠体细胞微核增加。

Objective To evaluate the effects of benzene on somatic cells genetic materials of mice. Methods There 50 Kunming mice were randomly divided into five groups： negative control, positive control and low, middle, high three dosage groups. The three dosage groups were treated with inhalation benzene 2 hours a day at the dose of 0.3g/m3,0.6g/m3,1.2g/m3. Fifteen days later, then the frequencies of PCEMN in sternum bone marrow were assessed. Remits Compared with negative control group, the frequencies of PCEMN were decreased significantly in all groups （ P 〈 0.05）, and a dose - dependant manner response was obvious observed. Condusion Benzene is capable of inducing the increase in frequencies of PCEMN of mice.