星形细胞瘤中^1H—MRS与PCNA抗原表达的相关研究

Correlation of ^1H-MRS features with PCNA expression in cerebral astrocytoma

目的:研究MRS、组织病理学与PCNA抗原表达的相关性。方法:58例经手术病理证实的星形细胞瘤患者,术前均行MR平扫,MRS及MR增强扫描,分别计算肿瘤不同区域的Cho/Cr,NAA/Cho代谢物比值,使用S-P免疫组化方法检测58例星形细胞瘤标本中PCNA抗原的表达。结果:高级别星形细胞瘤中Cho/Cr,Cho/NAA值显著高于低级别星形细胞瘤(P<0.05),各级别星形细胞瘤PCNA抗原表达与Cho/Cr比值有相关性(P<0.05)。结论:1H-MRS通过对NAA/Cho,Cho/Cr比值的定量分析,以及Cho/Cr比值与PCNA抗原表达的相关性分析,对星形细胞瘤术前恶性度分级以及间接的评价肿瘤细胞增殖潜能和预后都有重要的价值。

Objective： To study the correlation between ^1H-MRS, histopathology and PCNA expression. Methods： Fiftyeight patients with surgically and pathologically proved astrocytoma were analyzed. MR, ^1H-MRS and MR enhancement scan were performed before operation. The ratio of metabolic material （Cho/Cr, NAA/Cho） came from different areas of tumors were calculated respectively. PCNA was stained with S-P immunohistochemical technique in 58 samples of astrocytoma. Results： The ratios of metabolic material（Cho/Cr, Cho/NAA） of astrocytoma proved to be high pathological grade were significantly higher than those astrocytoma proved to be lower pathological grade （P〈0.05）. Correlation between PCNA expression of different grade astrocytoma and the ratio of Cho/Cr is good （P〈0.05）. Condusion： ^1H-MRS has important value both in assessing the malignant grading of astrocytoma before operation and in evaluating indirect proliferating potential and prognosis of tumor cells by quantitative analyzing the ratio of metabolic material （Cho/Cr, NAA/Cho）, and the correlation between the ratio of Cho/Cr and PCNA expression.