缺血预处理对兔缺血心肌的保护作用

Protective Effects of Ischemic Preconditioning against Myocardial Ischemic in Rabbits

采用动物缺血－再灌注模型，结扎兔冠状动脉左前降支（LAD）造成急性心肌梗塞（AMI）。实验组经过5min缺血，10min再灌注后持续缺血60min，3h再灌注；对照组直接造成60min缺血，3h再灌注。心肌梗塞范围由1％三苯四氮唑兰（TTC）染色确定，并以梗塞范围占缺血范围重量的百分比表示。结果：实验组梗死心肌明显少于对照组（P＜0.01）；实验组发生室性心律失常率明显低于对照组（P＜0．05）；实验组再灌注后血小板表面α－颗粒膜蛋白（GMP－140）分子数明显少于对照组（P＜0．05）。表明：预适应（Preconditioning，PC）具有心肌保护作用。

A model of acute ischemia-reperfusion injury was used in open-chest rabbits. All rabbits were randomly divided into two groups: Control and Preconditioned. Preconditioned rabbits(n = 8)were preconditioned with 5-min left anterior descending(LAD)occlusion following by 10-min reperfusion before the 60-min occlusion and 3-h reperfusion insult. Control (n = 8 ) rabhits were subjected to only the 60-min occlusion following by 3-hreperfusion insult. Infarct size was determined by 1% triphenyltetrazolium staining,and expressed as a percent of area at risk. Results: Infarct size was markedly reduced from 39. 5 ±6. 26 % in the control group to 7. 4 ±1.48%in preconditioned hearts (P <0.01); The incidence of ventricular arrhythmia was markedly reduced in preconditioned group (P<0. 05 ); The aggregation of platelet was limited in preconditioned rabblts (P<0.05 ).Conclusion: Preconditioning has protective effect on ischemic myocardium.