摘要
目的研究流行性乙型脑炎病毒在二倍体(2BS)细胞上的传代适应性,确定在2BS细胞上培养乙脑病毒的条件和方法。方法通过病毒毒力和免疫原性检测,观察在不同培养条件下乙脑病毒在2BS细胞上的增殖情况。结果pH值在7·4～7·6之间、人血白蛋白含量为0·2%、病毒稀释度为1:1000时,病毒维持液培养的病毒滴度最高。在2BS细胞上传50代的病毒滴度均在7·0logLD50以上,且差异无显著意义。连续传代后病毒的免疫原性下降。结论建议用于建立病毒种子库的流行性乙型脑炎病毒,在2BS细胞上传代不应超过3代。
Objective To study the adaptability of Japanese encephalitis virus （JEV） in diploid cells （2BS） and optimize the condition for culture of JEV in 2BS cells. Methods Observe the proliferation of JEV in 2BS cells under various conditions by virulence and immunogenicity tests. Results JEV reached a high titer after culture at a dilution of 1 ： 1 000 in maintenance medium （ pH 7.4- 7. 6） containing 0. 2% human serum albumin. The virus titers after subculture in 2BS cells for 50 passages were more than 7.0 logLD50 and showed no significant difference. The immunogenicity of JEV decreased significantly after continuous subculture. Conclusion It is suggest that the subculture of JEV for establishment of virus seed hank in 2BS cells shall be not more than 3 passages.
出处
《中国生物制品学杂志》
CAS
CSCD
2007年第7期500-502,共3页
Chinese Journal of Biologicals
关键词
流行性乙型脑炎病毒
二倍体细胞
传代
适应性
Japanese encephalitis virus（JEV）
Diploid cell
Subculture
Adaptability
