条斑紫菜多糖的分离纯化与抗肿瘤活性

Study on Isolation, Purification and Antitumor Activity of Polysaccharides from Porphyra yezoensis

目的:应用生化提纯技术从条斑紫菜(Porphyra yezoensis)中提取不同纯度的多糖,并观察各多糖组分对人乳腺癌细胞MCF-7生长的影响。方法:通过DEAE-52柱层析和Sephadex G-200柱层析,对条斑紫菜粗多糖热水提取物进行纯化;经HPLC,紫外和红外光谱对多糖各组分的纯度及结构进行初步鉴定;并用MTT法和流式细胞仪检测多糖对人乳腺癌细胞MCF-7生长的影响。结果:分离纯化出的各组分条斑紫菜粗多糖对人乳腺癌细胞MCF-7生长均有不同程度的抑制作用,其中PY-D2可诱导MCF-7细胞凋亡。结论:条斑紫菜多糖具有杀伤肿瘤细胞MCF-7的作用。

The polysaccharide from Porphyra yezoensis （PYP） was purified and analyzed by biochemical techniques. The effect of PEP on the proliferation of MCF-7 was investigated in vitro. Methods： The purified polysaccharide from Porphyra yezoensis was obtained by DEAE- cellulose and Sephadex G-200 column chromatography. The effect of PEP on the proliferation of MCF-7 was investigated in vitro by MTT method. Two components, PY-D1 and PY-D2, were isolated from the Porphyra yezoensis polysaccharide by ion-exchange chromatography DEAE-52. Two polysaccharides, PY-G1 and PY-G2, were further isolated from PY-D2 Ueda by gel filtration Sephadex G-200. PY-G was identified by UV and IR spectra. All of components could inhibit the proliferation of MCF-7. Results： It was found polysaccharides of Porphyra yezoensis had antitumor activity for MCF-7 cells.