紫外分光光度法测定柑桔皮中总黄酮的含量

QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN CITRUS PEEL BY ULTRAVIOLET SPECTROPHOTOMETRY

建立以芦丁为标准品的柑桔皮黄酮类含量测定方法,采用3种紫外—可见分光光度法对不同条件处理下的柑桔皮中总黄酮含量进行了测定和比较分析。研究表明,3种分光光度测定方法中,黄酮含量以硝酸铝显色法(345nm)为最高,直接测定法(270nm)次之,氯化铝显色法(415nm)最低;经过HPLC检测后,选定氯化铝(275nm)为显色系统的含量测定方法。回归方程为:Y=28.891X+0.0002,相关系数r=1,平均加样回收率为99.3%,RSD=1.84%(n=5),经不脱脂超声处理过的柑桔皮中所得黄酮含量为3.09%。该法操作简便,结果准确,重复性好,为柑桔皮中黄酮含量的测定提供了一种切实可行的途径。

Did establish a method which acted rutin as standard sample to measure determination of total flavonoids in citrus peel. Meanwhile, the contents of the total flavonoids in citrus peel that disposed through different conditions were measured and compared and analyzed by using the ultraviolet-visible spectrophotometry with three methods. The results showed that, of the three methods, the concentration of the total flavonoids was the highest by using the Al（NO3）3 （345 nm）, and the coloration method with direct measurement （270 nm） and AlCl3 （415 nm ） took the second and the third place. The method of assaying the total flavonoids was determined by the ultraviolet-visible spectrophotometry with AlCl3 （275 nm） system after being studied by HPLC. The regression equation was Y=28.891X＋0.000 2 with a relation coefficient r=1, the average recovery was 99.3 %, RSD =1.84 % （n=5）. The average content of the total flavonoids in non-isolating fat and ultrasonic treatment citrus peel was 3.09 %. This method was easily operated for measuring the total flavonoids in citrus peel. and accurate and reproducible,it could be a feasible way for measuring the total flavonoids in citrus peel.