青石棉体外诱发细胞微核与多核

Induction of micronuclei and multinuclei in vitro by crocidolite 1

采用国产青石棉对ＢＡＬＢ／ｃ－３Ｔ３细胞和Ｖ７９细胞染毒，发现该２种细胞均可被青石棉诱发微核与多核．并且观察到含青石棉细胞的微核细胞率和多核细胞率均显著高于不含青石棉的细胞，而后者又比阴性对照显著增高．这提示青石棉进入细胞是其发挥遗传毒性作用的重要条件，但并非必需条件．与青石棉样品比较（２２％），这２种细胞内所含青石棉长纤维（＞１０μｍ）比例增大（６０％－６６％），提示细胞对青石棉的吞噬或保留作用具有一定长度选择性．

BALB/c 3T3 and V79 cells were individually treated with crocidolite (made in China), and the frequencies of micronucleated and multinucleated cells were observed. The results demonstrated that in BALB/c 3T3 cells crocidolite induced increases of both micronucleated and multinucleated cells, although less potently than in V79 cells. It was also observed that the frequencies of both micronucleated and multinucleated cells in crocidolite containing cells were significantly higher than that in crocidolite free cells, and the latter was however higher than negative control. These indicate that the entry of crocidolite into cells is important, but not essential, for its genotoxicity. Furthermore, the proportion of long crocidolite fibers (>10 μm) contained in cells of both lines (60%-66%) was higher than that in crocidolite sample (22%, P<0.01), which indicates that the phagocytosis or retention for crocidolite fibers of the cells may be length related.