三种抗氧化剂对青石棉诱发微核,多核及转化细胞生长的影响

Effects of three antioxidants on crocidolite induced micronuclei and multinuclei and growth of transformed cells 1

在青石棉诱发Ｖ７９细胞微核与多核模型的基础上，以维生素Ｅ（ＶＥ），β－胡萝卜素（β－Ｃａｒ）或亚硒酸钠（Ｓｅ）分别与青石棉同时处理细胞，观察上述抗氧化剂对其微核细胞率与多核细胞率的影响．发现ＶＥ和Ｓｅ可分别降低其微核细胞率或多核细胞率，β－Ｃａｒ对二者均无影响．采用青石棉诱发的ＢＡＬＢ／ｃ－３Ｔ３恶性转化细胞，观察上述物质对其克隆形成率（ＣＦＥ）和细胞生长的影响．发现Ｓｅ，β－Ｃａｒ和ＶＥ分别在１０，６和２５０μｍｏｌＬ－１以上浓度出现ＣＦＥ降低，且细胞生长受抑．

Based on the model of induction of micronuclei and multinuclei by crocidolite in V79 cell line, the cells were treated with vitamin E (VE, 6.2-166.7 μmol·L 1 ), β carotene(β Car, 0.5-4.0 μmol·L 1 ), or sodium selenite(Se, 0.01-0.4 μmol·L 1 ), respectively, in combinations with crocidolite(0.62 μg·cm 2 ) for 24 h. The results indicated that VE had an inhibitory effect on the incidences of micronucleated cells, that Se reduced that of multinucleated cells in a narrow range of concentrations(0.04-0.08 μmol·L 1 ), and that β Car had no effect on that of either micronucleated or multinucleated cells. Transformed BALB/c 3T3 cells induced by crocidolite were used for the following study: the cells were treated with a series of doses of Se(10, 20 μmol·L 1 ), β Car(6, 12 μmol·L 1 ), or VE(250, 500 μmol·L 1 ), respectively, then the growth and colony formation efficiency of the cells were observed in different times (42-168 h). It was found that at the concentrations above 10, 6, or 250 μmol·L 1 , respectively, Se, β Car, or VE inhibited the growth and colony formation of the transformed cells. Of the three antioxidants, Se and β Car showed the above effects near their physiological concentrations in blood, but VE did not unless up to more than 10 folds of its physiological concentration.