胃癌及其癌前病变中ras P^(21)蛋白表达的定量研究

Quantitative analysis of the expression oi ras P^(21) oncoprotein in gastric cancer and precancerous lesion

目的:探索胃粘膜良、恶性病变中,细胞内rasP^(21)蛋白含量的变化规律与胃癌发生发展的关系.方法:应用图像分析系统对97例胃正常粘膜、各级异型增生和癌进行了抗ras基因产物P^(21)抗体免疫组化标记物的定量测定.结果:P^(21)阳性细胞胞浆平均吸光度值(A)在正常胃粘膜、轻、中、重度异型增生和高分化腺癌中分别为57.082、57.896、62.531、65.750、66.139,其中A在重度异型增生明显高于正常胃粘膜与轻度异型增生(P<0 .05～0.01),而与高分化腺癌接近(P>0.05).结论:提示P^(21)过表达与胃癌发生与分化程度可能有密切关系.图像分析定量测定能客观地反映胃粘膜不同病变中P^(21)蛋白的相对含量,对早期癌变细胞的检出有一定意义.

Objective:To explore the relationship of the expression of ras P21 in stomach and development ofgastric cancer, the expression of ras P21 oncoprotein was qualitatively analysed in gastric cancer and precan-cerous lesion. Methods: The amount of P21 has been assessed by immunohistochemical quantitative analysis using image analyzer in normal mucosa, precancerous lesion and cancer. Result: The percentage of positive cases of ras P21 increased gradually, in the gastric normal mucosa, mild, moderate, severe dysplasia and carcinoma, the absorption in cytoplasm of ras P21 is 57. 082, 57. 896, 62. 531, 65. 750, 66. 139 in the sequence of gastric normal mucosa, mild, moderate, severe dysplasia and well-differentiated adenocarcinoma. In severe dysplasia both the percentage of positivity and the absorption were significantly higher than that of normal mucosa and mild dysplasia (P<0. 05) , and approaching the expression level of well-differentiated adenocarcinoma (P>0. 05). Conclusion:The results show that over expression of P21 is related with the development and differentiation degree of gastric carcinoma. Quantitative analysis using image analyzer is capable of assaying the content of ras P21 objectively in various lesions of gastric mucosa, and thus useful for the early detection of canceration.