高效液相色谱法测定尿中儿茶酚胺及与荧光法的比较

Determination of Catecholamine in Urine by High Performance Liquid Chromatography with Electrochemical Detection and Comparison with Fluorinotric Method.

为比较高效液相色谱法与荧光法测定尿中儿茶酚胺，前者具有快速、灵敏、不受药物干扰、尿液用量少等优点。用高效液相色谱电化学检测器可同时测定尿中去甲肾上腺素（ＮＡ），肾上腺素（Ａ），多巴胺（ＤＡ）。酸化尿水解后，用乙酸乙酯抽提后，再经氧化铝吸附，用０．０５ｍｏｌ／ＬＨ３ＰＯ４洗脱，用反相离子对色谱。其变异系数分别为３．４％、３．１％、３．８％和３．０％，最小检出量分别为２５、５０和５０ｐｇ。尿中回收率分别为７１．７％～８６．０％、６３．５％～７１．５％和８３．２％～９７．１％。与三羟基吲哚法相比，相关系数为ｒ＝０．９８（ＮＡ）和ｒ＝０．８３（Ａ）。本法可代替荧光法，用于常规分析。

A high performance liquid chromatography with electrochemical detection(HPLC-EC) has been used to determine free urinary concentration of catecholamine(CA) in 20 normal subjects.Urine samples were prepurified with ethyl acetate before absorpted onto alumina.Revesed-phase ion-pair perpormance liquid chromatography technique showed that detection limits for NA、A、DHBA and DA were 25、50、25 and 50pg respectively.The coefficient of CA were 3.4%(NA), 3.1%(A), 3.8%(DHBA) and 3.0%(DA) respectively and recovery of CA were 71.7%～86.0%(NA), 63.5%～71.5%(A) and 83.2%～97.1%(DA).The results were compared with those of the trihydroxyindole procedure previously used.The correlations of NA and A by the two methods were r=0.98 and r=0.83.