SH3GL2在无病灶性难治性癫癎患者脑组织中的表达

Expressions of SH3GL2 mRNA and Protein in the Patients with Non-lesion Intractable Epilepsy

目的:分析无病灶性难治性癫患者脑组织中SH3GL2 mRNA与蛋白的表达水平,探讨可能的发病机制。方法:运用逆转录-聚合酶链式反应(RT-PCR)和Westernblot分别检测10例无病灶性难治性癫患者及10例对照组脑组织SH3GL2 mRNA及蛋白的表达。结果:RT-PCR检测显示,无病灶性难治性癫患者脑组织SH3GL2 mRNA表达的相对水平为(102.23±54.68),对照组为(44.59±17.12),两者差异有显著统计学意义(P<0.01);Westernblot分析表明,无病灶性难治性癫患者脑组织的SH3GL2蛋白表达相对水平(229.54±89.82)高于对照组(143.88±59.69),两者差异也有显著统计学意义(P<0.05)。结论:SH3GL2 mRNA与蛋白表达水平在无病灶性难治性癫患者脑组织中明显增强,提示表达于中枢神经系统的SH3GL2可能在人类难治性癫的发病机制中起了一定作用。

Aim： To analyze the levels of SH3GL2 mRNA and SH3GL2 in brain of the patients with nonlesion intractable epilepsy, and to explore a possible pathogenesis of this epilepsy. Methods： The levels of SH3GL2 mRNA and SH3GL2 in brain tissues taken from 10 cases of non-lesion intractable epilepsy patients and 10 cases of health controls were analyzed by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot, respectively. Results： RT-PCR showed that the relative levels of SH3GL2 mRNA expression in brain of the non-lesion intractable epilepsy patients and health controls were 102.23 ± 54.68 and 44.59 ± 17.12, respectively. There was a significant difference between of them （P 〈 0.01）. Western blot showed that the relative level of SH3GL2 in the non-lesion intractable epilepsy patients （229.54 ± 89.82） was distinct higher than that in the health controls （143.88 ± 59.69） （P 〈 0.05）. Conclusion： Both of SH3GL2 mRNA and SH3GL2 expressions in brain of the non-lesion intractable epilepsy patients were markedly increased, it was suggested that SH3GL2 may play an important role in the pathogenesis of non-lesion intractable epilepsy.