铜绿假单胞菌β-内酰胺类耐药相关基因检测

Resistant Genes of Beta-lactam Antibiotics from Clinical Isolates of Pseudomonas aeruginosa

目的了解临床分离铜绿假单胞菌(PAE)对β-内酰胺类抗菌药物的耐药并探讨其耐药基因的存在状况。方法对20株临床分离的PAE用微生物鉴定药敏仪检测阿莫西林、头孢噻肟、头孢吡肟、美罗培南、亚胺培南等9种β-内酰胺类抗菌药物的药敏表型,采用聚合酶链反应(PCR)法检测TEM、SHV、OXA、PER、GES、AmpC、IMP、VIM、oprD2等9种耐药基因,并与相关药敏表型比较。结果20株PAE中,β-内酰胺类耐药基因阳性率:TEM、DHA的阳性率为15%;SHV和OXA阳性率为10%;VIM和IMP的阳性率为5%;oprD2缺失率为25%,而PER、GES基因检测均为阴性。结论携带TEM、SHV、OXA、DHA、VIM、IMP以及oprD2基因缺失,是导致本组PAE对大多数β-内酰胺类抗菌药物耐药的主要机制。

OBJECTIVE To investigate β-1actamase coding genes and oprD2 gene in multidrug-resistant Pseudomonas aeruginosa isolated. METItOI）S Polymerase chain reaction（PCR） was used to detect various β-1actamase coding genes including TEM, SHV, OXA, PER, GES, IMP, VIM, plasmid type AmpC β-1actamase DHA, MIR and oprD2 in 20 strains of P. aeruginosa. RESULTS The detection rate of β-1actamase coding genes plasmid type AmpC, VIM, TEM, SHV, OXA and IMP were 15%, 5%, 15%, 10%, 10% and 5%, respectively, the loss rate of oprD2 was 25%, but PER and GES genes were negative. CONCLUSIONS The study indicated that the P. aeruginosa is carrying genes of DHA, TEM, SHV, OXA, VIM, IMP and being lost oprD2 gene, the latter is the essential resistance mechanism of P. aeruginosa to beta-lactam antibiotics in local area.