铜绿假单胞菌连续分离株耐药性与遗传学特征研究

Genetic Background and Antibiotic Resistance of Continuously Isolated Pseudomonas aeruginosa Strains

目的了解铜绿假单胞菌(PAE)连续分离株耐药性与遗传学特征。方法采用PCR检测86株PAE连续分离株耐药基因和整合子、转座子。结果86株中oprD2基因缺失47株(55.0%)、β-内酰胺酶基因blaCARB阳性26株(30.2%)、blaVIM阳性12株(14.0%)、blaDHA阳性3株(3.5%)、blaTEM阳性1株(1.1%);氨基糖苷类修饰酶基因ant(2″)-Ⅰ阳性24株(27.9%)、aac(6′)-Ⅱ阳性21株(24.4%)、aac(6′)-Ⅰb阳性13株(15.1%)、ant(3″)-Ⅰ阳性4株(4.7%),存在克隆传播现象。结论绍兴连续分离的PAEβ-内酰胺酶、氨基糖苷类修饰酶基因携带率高。PAE可导致克隆传播医院感染,并存在暴发性流行。

OBJECTIVE To investigate the genetic background and antibiotic resistance of continuously isolated P. aeruginosa strains in Shaoxing. METHODS Eighty six strains of P. aeruginosa were isolated from hospitalized patients, and drug-resistant genes, integrons and transposons were detected by PCR. RESULTS The positive rates of blaCARB, blaVIM, blaDHA, blaTEM, ant （ 2＂）- Ⅰ , aac （ 6＇）- Ⅱ , aac （ 6＇）- Ⅰ b and ant （3＂）- Ⅰ were 30. 2 %, 14.0;, 3. 5%, 1. 1;;, 27. 9%, 24. 4%, 15. 1; and 4. 7;. There were clone transmitted phenomenon. CONCLUSIONS There are very high positive percentages of blaCARB, blaVIM, ant（2r＇）- Ⅰ , aac（6＇）- Ⅱ , and aac （6＇）-Ⅰ b genes in continuously isolated P. aeruginosa strains. P. aeruginosa can induce clone transmitted hospital infection and it has fulminant prevalence.