不同基因型玉米某些氮代谢生理指标的差异研究

Study on Difference of Some Physiological Indexes of Nitrogen Metabolism in Maize of Different Genotypes

通过盆栽试验,在施氮(N+)和不施氮(N-)条件下,对不同基因型玉米穗位叶的叶绿素含量、硝酸还原酶活性和谷氨酰胺合成酶活性等生理指标进行了分析.结果表明,不同基因型材料的3项生理指标存在显著或极显著差异.在供试材料中,许178的叶绿素含量、硝酸还原酶活性和谷氨酰胺合成酶活性均最高,黄C最低,尤其在灌浆期比开花期表现更明显;增施氮肥可明显提高3项生理指标,但不同材料增幅不一,材料2(L2)对氮胁迫敏感,增施氮肥可明显提高其硝酸还原酶活性,而许178对氮胁迫不敏感,低氮条件下仍是氮高效利用自交系,为农大108成为氮高效品种提供了遗传基础.

Used pot cuitivation,an elite hybrid Nongda 108 and its parents as well as its three F2：3 families were grown under nitrogen （N） applied （ N ＋ ） and no N applied （N - ） conditions to analyze the chlorophyll content, nitrate reductase（NR） activity and glutamine synthetase（GS） activity of ear leaf in maize. The results showed that the three physiological indexes varied with genotypes significantly. All three indexes of the inbred Xu 178 was the highest, but inbred Huang C was lowest among the 6 testing materials, especially during the grain filling stage. Nitrogen input may increase the three indexes obviously, while the increase range varied with genotypes, material 2 （L2） was sensitive to low N stress and its NR activity could be increased obviously by adding N fertilizer, while Xu 178, still as a high nitrogen use efficiency genotype under low N condition, wasn＇ t sensitive to N stress, which offered heredity foundation for Nongda 108 as a high N use efficiency hybrid.