摘要
目的探讨高氧暴露新生大鼠肺组织结构的变化和连接蛋白Connexin26(Cx26)的表达情况及维甲酸对其影响。方法24只3日龄SD大鼠随机分为3组:高氧暴露维甲酸组(维甲酸组)、高氧暴露安慰组(高氧组)、空气暴露对照组(对照组);采用950mL/L氧体积分数条件下制作新生大鼠高氧肺损伤模型;观察大鼠一般情况,用HE染色法观察肺组织结构变化及辐射状肺泡计数(RAC),免疫组织化学检测Cx26的表达。结果体质量、RAC等方面与空气组相比,高氧组、维甲酸组均有显著差异[(13.53±1.27)、(13.38±1.29)、(17.37±0.89)g,F=30.19P=0;(11.15±1.33)、(12.49±1.47)、(13.94±0.98),F=9.59P=0];Cx26蛋白在空气组表达比较局限,高氧组弥散表达,而维甲酸组更多表达;阳性细胞分别为(13.68±1.28)%、(17.82±1.72)%、(19.69±1.77)%,F=29.36P=0。结论维甲酸对新生大鼠高氧肺损伤具有保护作用,可能与间隙连接蛋白Cx26表达的改变有密切的关系。
Objective To observe influence of retinoic acid on the lung damage and expression of Connexin26 (Cx26) of neonatal rats suf- fered from hyperoxia. Methods Twenty - four 3 - day SD rats were randomly divided into three groups : retinoic acid + hyperoxia, hyperoxia, air group;rats in the group of hyperoxia were suffered 7 days hyperoxia ( the level of oxygen was above 950 mL/L) ,air group was instead of air. All rats were weighed, value of radical alveolar counts ( RAC ) and microstructure of the lungs were observed by HE staining under light and electron microscope respectively;and the expression of Cx26 in the lungs was determined by immunohistochemistry. Results Compared with air group, weight,value of RAC were both decreased obviously in hyperoxia,retinoic acid + hyperoxia groups[ ( 13.53 ± 1.27 ), ( 13.38 ± 1.29) , ( 17.37 ± 0.89)g,F=30.19 P=0;11.154±1.33,12.49±1.47,13.94±0.98,F=9.59 P=0),while the expression of Cx26 increased [(13.68± 1.28 ) % , ( 17.82 ± 1.72) % , ( 19.69 ± 1.77 ) % , F = 29.36 P = 0]- Conclusion Retinoic acid has a protective effection from damaged lung of neonatal rats suffered from hyperoxia,and it may be related to the alteration of expression of Cx26 protein in the lungs.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第14期1094-1096,共3页
Journal of Applied Clinical Pediatrics