维甲酸对新生大鼠高氧肺损伤的干预作用

Interferon Effect of Retinoic Acid on Lung Damage of Neonatal Rats Suffered from Hyperoxia

目的探讨高氧暴露新生大鼠肺组织结构的变化和连接蛋白Connexin26(Cx26)的表达情况及维甲酸对其影响。方法24只3日龄SD大鼠随机分为3组:高氧暴露维甲酸组(维甲酸组)、高氧暴露安慰组(高氧组)、空气暴露对照组(对照组);采用950mL/L氧体积分数条件下制作新生大鼠高氧肺损伤模型;观察大鼠一般情况,用HE染色法观察肺组织结构变化及辐射状肺泡计数(RAC),免疫组织化学检测Cx26的表达。结果体质量、RAC等方面与空气组相比,高氧组、维甲酸组均有显著差异[(13.53±1.27)、(13.38±1.29)、(17.37±0.89)g,F=30.19P=0;(11.15±1.33)、(12.49±1.47)、(13.94±0.98),F=9.59P=0];Cx26蛋白在空气组表达比较局限,高氧组弥散表达,而维甲酸组更多表达;阳性细胞分别为(13.68±1.28)%、(17.82±1.72)%、(19.69±1.77)%,F=29.36P=0。结论维甲酸对新生大鼠高氧肺损伤具有保护作用,可能与间隙连接蛋白Cx26表达的改变有密切的关系。

Objective To observe influence of retinoic acid on the lung damage and expression of Connexin26 （Cx26） of neonatal rats suf- fered from hyperoxia. Methods Twenty - four 3 - day SD rats were randomly divided into three groups ： retinoic acid ＋ hyperoxia, hyperoxia, air group;rats in the group of hyperoxia were suffered 7 days hyperoxia （ the level of oxygen was above 950 mL/L） ,air group was instead of air. All rats were weighed, value of radical alveolar counts （ RAC ） and microstructure of the lungs were observed by HE staining under light and electron microscope respectively;and the expression of Cx26 in the lungs was determined by immunohistochemistry. Results Compared with air group, weight,value of RAC were both decreased obviously in hyperoxia,retinoic acid ＋ hyperoxia groups[ （ 13.53 ± 1.27 ）, （ 13.38 ± 1.29） , （ 17.37 ± 0.89）g,F=30.19 P=0;11.154±1.33,12.49±1.47,13.94±0.98,F=9.59 P=0）,while the expression of Cx26 increased [（13.68± 1.28 ） % , （ 17.82 ± 1.72） % , （ 19.69 ± 1.77 ） % , F = 29.36 P = 0]- Conclusion Retinoic acid has a protective effection from damaged lung of neonatal rats suffered from hyperoxia,and it may be related to the alteration of expression of Cx26 protein in the lungs.