精脒对CAOV3卵巢癌细胞端粒酶活性的影响

Effect of spermidine on telomerase activity of CAOV3 cells

目的:探讨精脒对CAOV3卵巢癌细胞的生长和端粒酶活性的影响。方法:应用MTT法观察精脒对CAOV3卵巢癌细胞生长的影响,应用TRAP-银染色法检测精脒对CAOV3卵巢癌细胞端粒酶活性的影响。结果:在细胞培养过程中,外源性增加精脒浓度可使细胞生长速度加快。用不同浓度精脒室温处理的CAOV3细胞端粒酶提取液2小时后,端粒酶活性与对照组差异无显著性。CAOV3细胞经不同浓度精脒处理3天后的端粒酶活性与对照组相比明显增高。结论:精脒浓度的升高可引起端粒酶活性的增高,精脒引起的细胞增殖与端粒酶活性增高有关。

Objective： To explore the effect of spermidine on the cell growth and the telomerase activity in CAOV3 cells. Methods： MTT assay was used to detect the effects of spermidine, and TRAP method was used to detect the telomerase activity in spermidine treating CAOV3 cells.Results：During cell growth, increase exogenous spermidine resulted in the increase rate of cell proliferation. Telomerase activity of CAOV3 cells telomerase extraction treated with spermidine for 2 hours had no significant difference from the control group. Telomerase activity of CAVO3 cells treated with spermidine for 3 days significantly increased compared to the control group. Conclusion： Telomerase activity increased with the increasing of spermidine levels. Spermidine inducing cell proliferation was related to the telomerase activity increasing.