病毒性心肌炎患儿血清白细胞介素-18、肿瘤坏死因子-α、干扰素-γ水平及其相关性

Study of Serum Interleukin-18,Tumor Necrosis Factor-Alpha,Interferon-Gama Level and Their Relationships in Children with Viral Myocarditis

目的研究细胞因子白细胞介素-18(IL-18)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)在病毒性心肌炎(VM)患儿血清中水平及相互关系。方法采用双抗体夹心ELISA方法检测72例VM患儿及25例健康儿童血清IL-18、TNF-α、IFN-γ水平。结果1.VM组血清中IL-18、TNF-α、IFN-γ水平高于正常对照组,均有显著性差异(Pa<0.05);2.VM组血清中IL-18与TNF-α呈直线正相关(r=0.35P<0.05)。结论细胞因子IL-18、TNF-α、IFN-γ参与VM发病免疫过程,IL-18具有抗病毒作用,与TNF-α同样具有心肌免疫损伤可能。

Objective To study the relationship and changes of cytokine interleukine - 18 （IL - 18 ）, tumor necrosis factor alpha （TNF -α） and interferon - gama（ IFN -γ） in viral myocarditis（VM）. Method Changes of serum cytokine IL - 18 ,TNF -α and IFN -γ of 72 acute VM patients and 25 healthy children were detected by enzyme - linked immunosorbent assay （ELISA）. Results 1. P - values represented the obvious significance of difference as comparing the concentration of serum cytokine IL - 18 ,TNF -α and IFN -γ of VM children with that of the control group （P 〈 0.05）. There was positive correlation between IL - 18 and TNF -α （ r = 0. 35 P 〈 0.05）. Conclusions Cytokine IL - 18, TNF -α and IFN -γ play role in the VM pathogenesis. Cytokine IL - 18 can not only resist virus but also cause immuno - injury like TNF -α in children with acute VM .