摘要
利用焦锑酸钾电镜定位细胞Ca2+的技术,及铅捕获法定位细胞Ca2+-ATPase的技术,研究小鼠精子获能及顶体反应过程中Ca2+及Ca2+-ATPase的变化,结果表明:获能前精子顶体囊外缘有Ca2+分布,顶体膨胀时,Ca2+消失,开始顶体反应时,顶体外膜,顶体囊泡及顶体后区有Ca2+分布,顶体反应后,仅顶体后区有Ca2+分布,精子尾部线粒体及轴丝始终有Ca2+分布。精子头部获能前有强的Ca2+-ATPase阳性反应,获能后减弱直至消失,而尾部细胞膜则一直有Ca2+-ATPase阳性反应。从结果推测:内源Ca2+为精子顶体反应的诱导物,外源Ca2+为精子顶体反应的维持物,而Ca2+-ATPase则在顶体反应前出现。
Ca 2+ and Ca 2+ ATPase were localized by potassium pyroantimonate and lead ion capture technique respectively to study the changes of Ca 2+ and Ca 2+ ATPase during mouse sperm capacitation and acrosome reaction.The results showed that:Ca 2+ positive precipitation distribute at the outer edge of uncapacitated sperm acrosome;when sperm initiated capacitation,Ca 2+ positive precipitation disappeared from the outer edge of acrosome.During sperm capacitation,the outer acrosomal membrane,acrosomal vesicles and postacrosome area showed Ca 2+ positive precipitation.After sperm underwent acrosome reaction,the outer acrosomal membrane shed and inner acrosomal membrane exposed,it did not show Ca 2+ positive precipitation,but the postacrosome area showed Ca 2+ positive precipitation yet.The mitochodria and axoname showed Ca 2+ positive precipctation in whole process.The uncapacitated sperm showed very strong Ca 2+ ATPase positive reaction,and became weaker at last.But sperm tail showed Ca 2+ ATPase positive reaction at cytoplasma membrane and axoname before and after acrosome reaction.The results showed that:endogenous Ca 2+ is the inducer of acrosome reaction,exogenous Ca 2+ is the maintainer of it,and Ca 2+ ATPase pump out Ca 2+ from sperm.
出处
《电子显微学报》
CAS
CSCD
1997年第2期87-92,共6页
Journal of Chinese Electron Microscopy Society
基金
中国科学院"八五"重点项目
关键词
精子获能
顶体反应
细胞生物学
小鼠
Ca 2+ \ Ca 2+ ATPase electron microscopic histochemistry\ Capacitation\ Acrosome reaction