罗非鱼内脏蛋白酶超声波提取工艺的研究

Study on Ultrasonic Extracting Process of Protease from Tilapia Viscera

本实验以罗非鱼加工废弃物——内脏为原料,研究提取蛋白酶的工艺方法,用单因素试验和正交试验研究确定了超声波法提取内脏中蛋白酶的最佳工艺条件。结果表明:从罗非鱼胃中提取胃蛋白酶的最佳工艺是:缓冲液pH1,提取温度为25℃,提取时间为60min,超声波强度为70W。从罗非鱼肠中提取蛋白酶的最佳工艺是:缓冲液pH7.5,提取温度为35℃,提取时间为85min,超声波强度为70W。为进一步研究和应用罗非鱼内脏蛋白酶提供了基础参数。

The method of extracting process of protease, the optimum ultrasonic extracting conditions of protease from viscera of tilapia which processing waste by single factor test with the orthogonal test design were studied. The results showed that the optimum conditions of pepsin from stomach of tilapia were： pill, 25 ℃, 60 rain, ultrasonic intensity 70 W. The optimum conditions of protease from intestines of tilapia were： 35 ℃, 85rain, pH 7.5, ultrasonic intensity 70 W. Further work should be carried out on the character of the enzymes and the applications.