摘要
Lactoferricin是一种天然抗菌肽。本实验进行了Lactoferricin基因的PCR合成,将其引入质粒pGEX-6p-1,转化E.coli JM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因表达载体pG-Lfn。
Lactoferricin is a kind of natural antibacterial peptide. In this study, the gene of Lactoferricin was synthesized with PCR, and was linked with the plasmid of pGEX-6p- 1, then transformed into E.coli JM- 109. The identifications of PCR, enzymical degestion and sequencing were carded out, and the expressing vector of pG-Lfn was successfully constructed.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第7期286-290,共5页
Food Science
