摘要
本研究以蜜蜂腹部为材料,通过研磨进行组织破碎,然后硫酸铵沉淀、High Q离子交换层析、疏水作用层析和Superdex G-75凝胶层析,得到的α-葡萄糖苷酶在SDS-PAGE上呈单一蛋白质条带,分子量约为55kDa。以4-硝基苯-α-D-吡喃葡萄糖苷(PNPG)为底物,研究酶催化反应动力学,结果表明:α-葡萄糖苷酶的最适反应pH范围为5.0～6.0,而pH稳定性范围为5.0～10.0。该α-葡萄糖苷酶的热稳定性比较差。其最适反应温度范围在35～45℃内。Zn2+、Mg2+、Mn2+和Fe2+对α-葡萄糖苷酶有激活作用。而Cu2+和Co2+对α-葡萄糖苷酶有抑制作用。酶动力学参数Km和Vmax分别为0.183mmol/L和0.085μmol/min·mg。
In this paper, α-glucosidase was purified to electrophoresis degree after being extracted from honey bee abdomen with ammmonium sulfate precipitation, with High Q chromatography, hydrophobic interaction chromatography and Superdex G- 75 chromatography. Its relative molecular weight is determined to be around 55 kDa by SDS-PAGE. The optimal temperature and pH range are 35 ~45 ℃ and 5.0~6.0, respectively, but the temperature stability is poor. The pH stability range is 5.0~ 10.0. Zn^2＋, Mg^2＋, Mn^2＋ and Fe~ can activate the enzyme, however, Cu^2＋ and Co^2＋ can cause inhibition, and glucose is also a strong inhibitor. The Vmax value is 0.085 la mol/min · mg and the Km value is 0.183 mmol/L for p-Nitrophenyl-α-D-glucopyranoside （PNPG）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第7期304-308,共5页
Food Science
