摘要
采用反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)和大气压化学电离质谱(APCI-MS)联用技术,分析赤芝中含有的三萜类化合物。使用反相C18色谱柱,以乙腈和水(含2%冰醋酸)为流动相梯度洗脱,流速为0.8ml/min,室温下,色谱检测波长253nm;用DAD检测器记录各个色谱峰的紫外吸收光谱,采用APCI-MS进行在线同步分析,记录TIC总离子流色谱图和各个色谱峰的质谱图,通过紫外光谱及质谱分析并与文献对照初步鉴定了赤芝中的32个三萜类成分。
High performance liquid chromatography (HPLC)-atmosphcric pressure chemical ionization-mass spectrometry (APCI-MS) was applied to analyze the triterpenoids in extracts of Ganoderma lucidum. The extraction conditions are: Hypersil C4 (4.6 mm × 150 ram) column with gradient elution with acetonitrile and water (2.0% acetic acid) as mobile phase, flow-rate 0.8 ml/min, and room temperature about 25℃. The detection wavelength is 253 nm and the Lrv spectra of peaks are obtained with a photodiode array detector (DAD). On-line APCI-MS in positive and negative mode was used to get the mass spectrum of the analyte. Thirty-two components of Ganoderma lucidum are primarily identified by comparison of the UV spectra and mass spectrum according to published literatures.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第7期367-372,共6页
Food Science
基金
青岛"2004将才计划"项目(04-3-JJ-11)
共建生物医药研发测试中心资助项目(LS-05-KJZX-76)