高效液相色谱-大气压化学电离质谱法分析赤芝中的三萜类成分

Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS

采用反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)和大气压化学电离质谱(APCI-MS)联用技术,分析赤芝中含有的三萜类化合物。使用反相C18色谱柱,以乙腈和水(含2%冰醋酸)为流动相梯度洗脱,流速为0.8ml/min,室温下,色谱检测波长253nm;用DAD检测器记录各个色谱峰的紫外吸收光谱,采用APCI-MS进行在线同步分析,记录TIC总离子流色谱图和各个色谱峰的质谱图,通过紫外光谱及质谱分析并与文献对照初步鉴定了赤芝中的32个三萜类成分。

High performance liquid chromatography （HPLC）-atmosphcric pressure chemical ionization-mass spectrometry （APCI-MS） was applied to analyze the triterpenoids in extracts of Ganoderma lucidum. The extraction conditions are： Hypersil C4 （4.6 mm × 150 ram） column with gradient elution with acetonitrile and water （2.0% acetic acid） as mobile phase, flow-rate 0.8 ml/min, and room temperature about 25℃. The detection wavelength is 253 nm and the Lrv spectra of peaks are obtained with a photodiode array detector （DAD）. On-line APCI-MS in positive and negative mode was used to get the mass spectrum of the analyte. Thirty-two components of Ganoderma lucidum are primarily identified by comparison of the UV spectra and mass spectrum according to published literatures.