摘要
目的:建立一种可靠的测定番茄及其制品中番茄红素含量的C18-HPLC-PDA方法。色谱条件:固定相为DIAMONSILTM柱(Dikma Technologies,250×4.6mm,5μm);流动相A为乙腈:水(9:1);流动相B为乙酸乙酯;线性梯度洗脱:在15min内,流动相B由0%增为100%(V/V),随后5min,流动相B保持100%;流速1.0ml/min,PDA波长范围260～600nm;色谱图检测波长471nm;进样量20μl;柱温=室温。结果:此方法的定量限为0.10μg/ml,质量浓度在0.10～25.60μg/ml范围内浓度与组分峰面积的线性相关系数为0.9994,RSD为2.6%,回收率为99.3%。结论:这是一种番茄及其制品中番茄红素含量测定的可靠方法。
Objective： This study aims to develop a reliable quantitative detection method of lycopene from tomato and processed products by C18-HPLC-PDA. Chromatographic conditions： stationary phase DIAMONSILTM column （Dikrna Technologies, 250 × 4.6mm, 5 μm）; mobile phase A acctonitrilc-water （9：1 ）; mobile phase B ethyl acetate; linear gradient： an increased mobile phase B from 0 to 100% （V/V） in 15 minutes followed by 100% B for 5 minutes; flow rate 1.0 ml/min; PDA wavelength range 260~ 600 nm; monitoring wavelength on chromatograrn 471 nm; injection volume 20 μl; column temperature： room temperature. Results： Minimum detection limit of this method is 0.10 μg/ml. In the range of 0.10~25.60 μg/ml, linear correlation coefficient between the concentration and peak area of lycopcnc fraction 0.9994 RSD 2.6%, recovery 99.3%. Conclusion： This method is reliable to detect lycopenc amount from tomato and processed products quantitatively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第7期453-456,共4页
Food Science
基金
北京市自然科学基金对外合作项目(SZ200511417020)
