黑果枸杞色素的毒理学研究

Toxicological Assessment of Pigment of Lycium ruthenicum Murr.

目的:研究黑果枸杞色素作为食用色素使用的毒理学安全性。方法:利用小鼠急性毒性试验、Ames试验、骨髓细胞微核试验、小鼠精子畸变试验和30d喂养试验进行毒理学研究。结果:急性毒性试验表明,黑果枸杞色素的KM小鼠LD50大于24g/kgbw,说明该受试物属于无毒级物质。遗传毒性试验:Ames试验、骨髓细胞微核试验、小鼠精子畸变试验的结果皆为阴性,未显示出致突变性。而30d喂养试验表明,黑果枸杞色素在2.4、1.2、0.24g/kgbw剂量时,对动物的身体、脏器的生长发育以及血液生化指标等均无明显不良影响。结论:黑果枸杞色素具有较好的食用安全性,可以作为药食两用天然色素使用。

Objective： The paper studied the toxicological safety of the pigments of Lycium ruthenicum Murr. Method： The toxicological safety properties were examined by tests of acute toxicity, Ames test, bone marrow cell micronucleus, sperm shape abnormality test, thirty days feeding test. Results： According to the result of acute toxicity LD50 test in mice, the LD50 of pigment ofLycium ruthenicum Murr. was more than 24 g/kg bw, which fell into the scale of non-toxicity. The negative results were observed in the genetic toxiticy test of Ames experiment, bone marrow cell micronucleus, sperm shape abnormality test. Abnormal phenomena had not been found in 30 days feeding test under the experimental dosage range of 2.4, 1.2, 0.24 g/kg bw. Conclusion： The pigment of Lycium ruthenicum Murr. has good edible-safety properties, can be used widely as natural food plantpigment.