麦胚蛋白酶解物对小鼠抗氧化作用的研究

Antioxidation Effects of Wheat Germ Protein Hydrolysates on Mice

研究不同剂量麦胚蛋白酶解物WGPHs对小鼠抗氧化作用的影响。80只昆明系小鼠,雌雄各半,分为基础饲料对照大组和高脂饲料对照大组,各大组随机分为四小组,分别为对照组、WGPHs低剂量组、WGPHs中剂量组、WGPHs高剂量组。对照组灌胃生理盐水,其余三组分别按100、500、1000mg/kg·d的剂量灌胃WGPHs水溶液。各组连续灌胃30d后,处死小鼠,检测小鼠血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)含量。结果表明:WGPHs在显著提高小鼠血清SOD和GSH-Px活性(p<0.05)的同时能显著降低血清MDA含量(p<0.05),其作用效果随着WGPHs剂量的增加而增大,WGPHs可增强小鼠的抗氧化作用。

To study the effects of wheat germ protein hydrolysates（WGPHs） in different doses on the serum antioxidant activities in mice. Eighty Kunming mice were divided into two big groups of normal fat feed and high fat feed, one big group were divided into 4 groups： control group, low dose of WGPHs group, medium dose of WGPHs group and high dose of WGPHs group. The mice supplemented with physiological saline and WGPHs at 100, 500, 1000 mg/kg · d respectively by gavage one time a day. The mice were euthanatized after 30 days. The activities of SOD,GSH-Px and the level of MDA were determined. The results showed that the content of MDA in the serum reduced （p 〈 0.01）, the activities of SOD and GSH-Px increased （p 〈 0.01） significantly in the serum; The activities of SOD, GSH-Px increased while the contents of MDA decreased in the experimental group dose-dependently. WGPHs can promoted the abilities of antioxidation of mice.