碱性成纤维细胞生长因子对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK/ERK、MKP-1通路的影响

Effects of basic fibroblast growth factor on the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line and the signaling pathway of MEK/ERK and MKP-1

目的:观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK1/2、ERK1/2及MKP-1表达的影响。方法:培养人涎腺腺样囊性癌细胞株(ACC-2),MTT比色法测定不同浓度bFGF对细胞增殖的影响;免疫沉淀法纯化蛋白并ERK试剂盒测定ERK活性;免疫印迹法测定p-MEK1/2、p-ERK1/2及MKP-1表达。结果:MTT实验显示bFGF明显增强ACC-2细胞增殖,免疫沉淀法显示bFGF上调ERK活性,免疫印迹法显示bFGF明显增强p-MEK1/2、p-ERK1/2表达及抑制MKP-1表达。结论:bFGF可促进人涎腺腺样囊性癌ACC-2细胞株增殖,其途径与上调ERK活性、激活MEK/ERK通路、抑制MKP-1表达有关。

Objective To investigate the effects of basic fibroblast growth factor（bFGF） on the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line and the signaling pathway of MEK/ERK and MKP-1 in vitro. Methods The effect of bFGF on proliferation of ACC-2 cell line was observed by MTY assay. ERK activity was measured by immuno-precipitation, p-MEK1/2, p-ERK1/2 and MKP-1 expressions were assessed by Western blot. Results bFGF could enhance the proliferation of ACC-2 cell line. The proliferation ratio of ACC-2 cell line stimulated by bFGF increased significantly. The intracellular ERK activity, p-MEK1/2 and p-ERK1/2 expressions were up-regulated and MKP-1 expression was down-regulated by bFGF. Conclusion bFGF promotes the proliferation of ACC-2 in the dose dependent manner, of which the possible mechanism may be associated with the up-regulation of MEK/ERK signaling pathway and the down-regulating of MKP-1 expression.