促愈颗粒对大鼠乙酸胃溃疡愈合质量的影响

Effect of Cuyu granule on the quality of ulcer healing in tats with gastric ulcer induced by acetic acid

目的:观察促愈颗粒对大鼠乙酸胃溃疡愈合质量的影响.方法:用冰醋酸制备大鼠慢性胃溃疡模型,所有大鼠随机分为4组(空白模型组、促愈颗粒组、雷尼替丁组和正常对照组).HE染色观察大鼠愈合性胃溃疡再生黏膜腺体成熟度和炎症细胞浸润情况;硝酸还原法检测血清NO含量;放射免疫法检测血浆PGE2含量;免疫组织化学技术检测大鼠胃黏膜EGF的表达情况;透射电镜观察大鼠再生黏膜超微结构的变化.结果:给药28d后与模型组和雷尼替丁组比较,促愈颗粒组再生黏膜囊性扩张腺体数量及炎细胞数量明显减少(再生黏膜囊性扩张腺体数量:1.43±0.53 vs 3.84±1.08.2.36±0.79,P<0.01和P<0.05;炎细胞数量:9.92±2.66 vs 28.32±6.96,17.92±4.76,P<0.01和P<0.05);血清NO及血浆PGE2含量显著增高(NO:105.41±8.02μmol/g vs 67.35±16.85,79.1 8±28.05μmol/g,P<0.0 1或P<0.05;PGE2:125.50±11.95 ng/L vs 97.33±11.84,118.83±13.25 ng/L,P<0.01和P>0.05);胃黏膜EGF表达明显增强(25.38±5.17 vs 16.82±2.13,21.12±6.08,P<0.05和P<0.05);促愈颗粒组超微结构的恢复亦优于模型组和雷尼替丁组.结论:促愈颗粒能提高溃疡再生黏膜结构,功能成熟度及电镜下成熟度,从而提高溃疡愈合质量.

AIM： To investigate the effect of Cuyu granule （CYG） on the quality of ulcer healing （QOUH） in rats. METHODS： Rat model of chronic gastric ulcer was induced by acetic acid and all the rats were randomly divided into 4 groups, named group A （control）, B （model）, C （treated with ranitidine） and D （treated with CYG）, respectively. The numbers of distended glands and inflammatory cells of healed gastric mucosa in rats were observed by HE staining, and the contents of nitric oxide （NO） in serum and prostaglandin E2 （PGE2） in plasma were determined by the nitric acid reductase process and radioimmunoassay （RIA） respectively. The protein expression of epidermal growth factor （EGF） was examined by immunohistochemistry, and the ultra-structure of regenerative mucosa was observed by transmission electronic microscopy. RESULTS： Twenty-eight days after drug administration, the numbers of distended glands and inflammatory cells in group D were significantly decreased in comparison with those in group B and C （distended glands： 1.43 ± 0.53 vs 3.84 ± 1.08, 2.36 ± 0.79, P 〈 0.01 and P 〈 0.05; inflammatory cells： 9.92 ± 2.66 vs 28.32 ± 6.96, 17.92 ± 4.76, P 〈 0.01 and P 〈 0.05）; The levels of serum NO and plasma PGE2 were markedly higher in group D that those in group B and C （NO： 105.41 ± 8.02 μmol/g vs 67.35 ± 16.85, 79.18 ±28.05 μmol/g, P 〈 0.01 and P 〈 0.05; PGE2：125.50 ± 11.95 ng/L vs 97.33 ± 11.84, 118.83 ± 13.25 ng/L, P 〈 0.01 and P 〉 0.05）; The expression of mucosal EGF was also increased in group D （25.38 ± 5.17 vs 16.82 ± 2.13, 21.12 ± 6.08, both P 〈 0.05）; The ultra-structural restoration was also superior to the other two groups. CONCLUSION： CYG can ameliorate the structure of regenerative gastric mucosa and enhance the maturity of mucosal function and ultrastructure.