摘要
目的:探讨正常胃黏膜、不典型增生胃黏膜及胃癌组织中Caveolin-1,nm23及MMP-2蛋白的表达.方法:应用免疫组化S—P法检测25例正常胃黏膜,65例不典型增生胃黏膜及71例胃癌组织中的Caveolin-1,nm23及MMP-2蛋白的表达.结果:正常胃黏膜、不典型增生胃黏膜及胃癌组织中,Caveolin-1和nm23阳性率有递减趋势,组间差异性有统计学意义(x^2=106.303,P〈0.05;x^2=64.254,P〈0.05);MMP.2蛋白阳性表达率有递增趋势,组间差异性有统计学意义(x^2=35.247,P〈0.05).Fisher精确概率检验显示在不同的浸润深度和淋巴结转移Caveolin-1,nm23及MMP-2阳性表达率组内差异有统计学意义(P〈0.05),而在年龄、性别和脉管侵犯组内差异无统计学意义(P〉0.05).Spearman等级相关分析显示Caveolin-1与nm23表达呈正相关(r=0.957,P〈0.05),Caveolin-1与MMP-2表达呈负相关(r=-0.975,P〈0.05),nm23与MMP-2表达呈负相关(r=-0.987,P〈0.05).结论:Caveolin-1与nm23的缺失以及MMP-2的过表达可能是胃癌发生、发展以及浸润转移的重要原因之一,Caveolin-1可以作为一种候选抑癌基因.
AIM: To investigate the protein expression of Caveolin-1, nm23 and matrix metalloproteinase-2 (MMP-2) in normal gastric rnucosa, gastric atypical hyperplasia and gastric carcinoma tissues.
METHODS: S-P irnmunohistochemical method was used to detect the expression of Caveolin-1, nm23 and MMP-2 proteins in normal gastric mucosa (n = 25), gastric atypical hyperplasia (n = 65), and gastric carcinomas (n = 71).
RESULTS: Caveolin-1 and nm23 expression showed a decreasing tendency in normal gastric mucosa, atypical hyperplasia and gastric cancer tissues ordinarily, and there were statistical dif- ferences between groups (x^2 = 106.303, P 〈 0.05; 2 = 64.254, P 〈 0.05). However, MMP-2 expression exhibited a increasing tendency (2 = 35.247, P 〈 0.05). Fisher's exact test demonstrated that the expression of Caveolin-1, nm23 and MMP-2 protein were significantly different between the cases with different invasion depths or lymph node metastases (P 〈 0.05), but not between the cases with different ages, sex and vessel invasions (P 〉 0.05). Spearman rank correlation analysis indicated that the expression of Caveolin-1 was positively correlated with that of nm23 (r = 0.957, P 〈 0.05), but negatively correlated with that of MMP-2 (r = -0.975, P 〈 0.05). Furthermore, there was a negative correlation between nm23 and MMP-2 expression (r = -0.987, P 〈 0.05).
CONCLUSION: The absent expression of Caveolin-1 and nm23 together with over-expression of MMP-2 may be one of the important causes for the onset, development and progression of gastric carcinoma. Caveolin-1 may serve as a tumor suppressor gene.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第15期1725-1730,共6页
World Chinese Journal of Digestology
基金
全军医药卫生科研基金
No.06MB102
甘肃省自然科学基金资助课题
No.3ZS061-A25-094~~