胃癌组织中Caveolin-1，nm23及MMP-2的表达及其意义

Expressions and significances of Caveolin-1,nm23 and matrix metalloproteinase-2 in gastric carcinomas

目的：探讨正常胃黏膜、不典型增生胃黏膜及胃癌组织中Caveolin-1，nm23及MMP-2蛋白的表达．方法：应用免疫组化S—P法检测25例正常胃黏膜，65例不典型增生胃黏膜及71例胃癌组织中的Caveolin-1，nm23及MMP-2蛋白的表达．结果：正常胃黏膜、不典型增生胃黏膜及胃癌组织中，Caveolin-1和nm23阳性率有递减趋势，组间差异性有统计学意义（x^2=106．303，P〈0．05；x^2=64．254，P〈0．05）；MMP．2蛋白阳性表达率有递增趋势，组间差异性有统计学意义（x^2=35．247，P〈0．05）．Fisher精确概率检验显示在不同的浸润深度和淋巴结转移Caveolin-1，nm23及MMP-2阳性表达率组内差异有统计学意义（P〈0．05），而在年龄、性别和脉管侵犯组内差异无统计学意义（P〉0．05）．Spearman等级相关分析显示Caveolin-1与nm23表达呈正相关（r=0．957，P〈0．05），Caveolin-1与MMP-2表达呈负相关（r=-0．975，P〈0．05），nm23与MMP-2表达呈负相关（r=-0．987，P〈0．05）．结论：Caveolin-1与nm23的缺失以及MMP-2的过表达可能是胃癌发生、发展以及浸润转移的重要原因之一，Caveolin-1可以作为一种候选抑癌基因．

AIM： To investigate the protein expression of Caveolin-1, nm23 and matrix metalloproteinase-2 （MMP-2） in normal gastric rnucosa, gastric atypical hyperplasia and gastric carcinoma tissues. METHODS： S-P irnmunohistochemical method was used to detect the expression of Caveolin-1, nm23 and MMP-2 proteins in normal gastric mucosa （n = 25）, gastric atypical hyperplasia （n = 65）, and gastric carcinomas （n = 71）. RESULTS： Caveolin-1 and nm23 expression showed a decreasing tendency in normal gastric mucosa, atypical hyperplasia and gastric cancer tissues ordinarily, and there were statistical dif- ferences between groups （x^2 = 106.303, P 〈 0.05; 2 = 64.254, P 〈 0.05）. However, MMP-2 expression exhibited a increasing tendency （2 = 35.247, P 〈 0.05）. Fisher＇s exact test demonstrated that the expression of Caveolin-1, nm23 and MMP-2 protein were significantly different between the cases with different invasion depths or lymph node metastases （P 〈 0.05）, but not between the cases with different ages, sex and vessel invasions （P 〉 0.05）. Spearman rank correlation analysis indicated that the expression of Caveolin-1 was positively correlated with that of nm23 （r = 0.957, P 〈 0.05）, but negatively correlated with that of MMP-2 （r = -0.975, P 〈 0.05）. Furthermore, there was a negative correlation between nm23 and MMP-2 expression （r = -0.987, P 〈 0.05）. CONCLUSION： The absent expression of Caveolin-1 and nm23 together with over-expression of MMP-2 may be one of the important causes for the onset, development and progression of gastric carcinoma. Caveolin-1 may serve as a tumor suppressor gene.