清肠泡腾栓对溃疡性结肠炎模型大鼠细胞因子的影响

Effect of Qingchang Effervescence Suppository on Ulcerative Colitis Models Rats

目的探讨中药清肠泡腾栓治疗溃疡性结肠炎的免疫学疗效机制。方法实验分设正常对照组、模型对照组、空白泡腾栓组、清肠泡腾栓组、清肠栓组、柳氮磺胺吡啶栓组共6个组,ELISA法检测血清、结肠上清肿瘤坏死因子-α(TNF-α)、白细胞介素-13(IL-13);免疫组化、原位杂交检测白细胞介素8(IL-8)mRNA阳性表达。结果模型对照组血清TNF-α较正常对照组升高(P<0.05),结肠黏膜IL-8 mRNA阳性表达明显增强(P<0.01);清肠泡腾栓明显降低结肠上清TNF-α含量以及IL-8 mRNA阳性表达,与模型对照组比较差异有显著性意义(P<0.05或P<0.01);清肠泡腾栓对IL-13的调控作用不明显,与模型对照组比较差异无显著性意义(P>0.05)。结论清肠泡腾栓通过抑制促炎细胞因子而对溃疡性结肠炎产生治疗作用。

Objective To study the immune mechanism of Qingchang Effervescence Suppository for ulcerative colitis （UC）. Methods All the rats were divided into normal control, model control, blank effervescence suppository, Qingchang Effervescence Stippository, Qingchang Suppository and Sulfasalazine groups. Tumor necrosis factor-α （TNF-α） and interleukin-13 （IL-13） in serum and colon supernatant were detected by enzyme-linked immunospecific assay. The expression of interleukin-8 （IL-8） mRNA was detected by immunohistochemical method and in situ hybridization. Results As compared with normal control group, TNF-α in serum and the expression of IL-8 mRNA in colon mucosa of model control group increased significantly （P〈0.05 or P〈0.01）. As compared with model control group, Qingchang Effervescence Suppository could reduce TNF-α and the expression of IL-8 mRNA significantly （P〈0. 05 or P〈0.01）, but had no obvious effect on IL-13 （P〉0.05）. Conclusion Qingchang Effervescence Suppository has therapeutic effect on UC by inhibiting proinflammatory cytokine.