Survivin基因在甲状腺癌中的表达及意义

Expression of survivin gene in thyroid carcinoma and its significance

目的研究 Survivin 基因在甲状腺癌中的表达及意义。方法应用免疫印迹法(Westernblotting),逆转录-聚合酶链反应(RT-PCR)法检测98例甲状腺癌及相应的癌旁组织中Survivin 基因的表达,应用原位末端标记法(TUNEL)检测甲状腺癌细胞凋亡指数(AI)。结果甲状腺癌组织表达 Survivin 蛋白与 RT-PCR 的结果一致,68.37%的甲状腺癌组织表达 Survivin 蛋白和mRNA,而癌旁组织内无一例阳性表达。其阳性表达率与病理分型、淋巴结转移、血行转移密切相关。所有甲状腺癌组织标本中均可检测凋亡细胞,Survivin 表达阳性组平均 AI(0.48±0.38)显著低于阴性组 AI(2.98±0.12,P=0.0082)。结论 Survivin 基因在甲状腺癌组织中异常表达;其表达与分化程度、淋巴结转移、血行转移密切相关;Survivin 对凋亡的抑制可能是其参与肿瘤发生、发展、转归的关键因素,Survivin 基因将成为甲状腺癌基因治疗的新靶点。

Objective To study the expression of survivin gene in thyroid carcinoma and its significance. Methods Samples of thyroid carcinoma were collected from 98 patients during operation. Western blotting and RT-PCR were used to detect mRNA and protein expression of survivin in the carcinoma tissue and the tissue around the carcinoma. TUNEL method was used to detect the apoptotic index （AI）. Results Westernblotting and RT-PCR methods showed similar results. The positive expression rates of survivin protein and mRNA in the thyroid carcinoma were both 68. 37% ; however, survivin was not expressed in all of the tissues around the thyroid carcinoma at both the mRNA and protein levels. The expression rate of survivin was closely related to the pathological typing, lymph node metastasis, and hematogenous metastasis. Apoptotic cells were found in all thyroid carcinoma. The mean A! of the surviving positive group was 0.48±0.38, significantly lower than that of the survivin negative group （2.98 ±0.12, P =0.0082 ）. Conclusion Survivin gene is expressed in most of the thyroid carcinoma. The positive expression rate of survivin is significantly linked to cell proliferating activity, lymph node metastasis, and hematogenous metastasis. Survivin gene will be a new target of gene therapy in thyroid carcinoma.